FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0120_pe_30.35100000046aff.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0120_pe_30.35100000046aff.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences222351
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGTGTGCATCTCGTATGCCGTC95714.304455567998345No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCGTGTGCATCTCGTATGCCGT11260.5064065374115699No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA4830.2172241186232578No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCGTGTGCATCTCGTATGCCG3600.16190617537137228No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3030.13627103093757167No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2910.1308741584251926No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2860.12862546154503465No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA2810.1263767646648767No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2590.11648249839218174No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA2300.10344005648726562No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC10600.044.3632145
ATGCCGT11600.040.5387944
TATGCCG12100.038.86363643
CGTATGC12300.038.41463541
CTCGTAT12300.038.41463539
GTATGCC12250.038.38775342
CGTGTGC12150.038.33333230
GACTCGT12450.038.1325326
ATCTCGT12400.038.1048437
GTGCATC12250.038.02040533
CCACGAG12550.038.0079720
AGACTCG12500.037.9825
CATCTCG12450.037.9518136
ACTCGTG12350.037.89473327
CACGAGA12600.037.85714321
CGAGACT12600.037.85714323
ACGAGAC12600.037.85714322
GCATCTC12500.037.835
CTCGTGT12350.037.71254728
CCCACGA12750.037.41176219