FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0120_pe_29.35100000046ad5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0120_pe_29.35100000046ad5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100043
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGTGTGCATCTCGTATGCCGTC1970019.691532640964386No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCGTGTGCATCTCGTATGCCGT40544.052257529262417No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCGTGTGCATCTCGTATGCCG8390.8386393850644223No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTT6220.6217326549583679No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTT5420.5417670401727258No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACTGTCTCTTA5200.5197764961066742No Hit
TATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTTA4710.47079755705046833No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAGAGTACTTTTTCTGTCTC3400.3398538628389792No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAGAGTACTTTTTCTGTCTCT3130.312865467848825No Hit
AAAAAGTACTCTGCGTTGATACCACCCGTACTCTGCGTTGATACTGTCTCT3070.3068680467399018No Hit
AAAAAGTACTCTGCGTTGATACCACCCGTACTCTGCGTTGATACCTGTCTC3050.30486890637026076No Hit
TTATACACATCTCCGAGCCCACGAGACTCGTGTGCATCTCGTATGCCGTCT2890.28887578341313236No Hit
AAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACTGTCTCTTATA2790.2788800815649271No Hit
AAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTTAT2600.2598882480533371No Hit
TATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTCTGTCTCTTATA2600.2598882480533371No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA2150.21490758973641333No Hit
GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA2090.20891016862749018No Hit
GAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTC2060.20591145807302857No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTCTGTCTCTTAT2010.20091360714892598No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCCTGTCTCT1820.18192177363733594No Hit
AAAAAGTACTCTGCGTTGATACCCGTACTCTGCGTTGATACCTGTCTCTTA1730.1729256419739512No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCCTGTCTCTTATACACATCTC1700.1699269314194896No Hit
GGTATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCT1640.16392951031056646No Hit
GTATCAACGCAGAGTACGGGTATCAACGCAGAGTACTTTTTCTGTCTCTTA1610.16093079975610486No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC1540.15393380846236118No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC1440.14393810661415593No Hit
TATCAACGCAGAGTACGGGTATCAACGCAGAGTACTTTTTCTGTCTCTTAT1440.14393810661415593No Hit
AAAAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGAGCC1380.13794068550523275No Hit
GTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCC1370.13694111532041223No Hit
AAAAAGTACTCTGCGTTGATACCCGTACTCTGCGTTGATACTGTCTCTTAT1300.12994412402666855No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAGAGTACTTTCTGTCTCTTA1180.1179492818088222No Hit
GGTATCAACGCAGAGTACGGGTGGTATCAACGCAGAGTACTTTTTCTGTCT1130.11295143088471957No Hit
AAAGTACTCTGCGTTGATACCCGTACTCTGCGTTGATACTGTCTCTTATAC1090.10895315014543747No Hit
GTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCG1070.10695400977579642No Hit
AAAGTACTCTGCGTTGATACCCGTACTCTGCGTTGATACCTGTCTCTTATA1050.10495486940615534No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACCTGTCTCTTATACACATCTCCG1030.10295572903651429No Hit
AAAGTACTCTGCGTTGATACCACCCGTACTCTGCGTTGATACTGTCTCTTA1020.10195615885169376No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC21800.043.24541545
GCCGTCT850.037.05882345
ATGCCGT26650.035.37523744
GCATCTC27500.034.6909135
CATCTCG27500.034.6090936
TGCATCT27650.034.58408734
GTGCATC27600.034.5652233
TGTGCAT27750.034.5405432
ATCTCGT27600.034.48369637
TCTCGTA27600.034.48369638
GTGTGCA27950.034.3738831
CGTGTGC28100.034.35053330
ACGAGAC28000.034.31250422
GTATGCC27750.034.29729542
CCACGAG28150.034.2895220
CACGAGA28150.034.2895221
CGAGACT28150.034.2895223
GAGACTC28150.034.2895224
AGACTCG28100.034.27046225
TATGCCG27650.034.2585943
Produced by FastQC (version 0.11.2)