FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0120_pe_28.35100000046abb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0120_pe_28.35100000046abb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences380671
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGTGTGCATCTCGTATGCCGTC351389.230542909756718No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCGTGTGCATCTCGTATGCCGT91942.415208933698653No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTT26870.7058588650041637No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACTGTCTCTTA23410.614966729800799No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTT20090.5277523110507498No Hit
TATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTTA18760.49281400474425424No Hit
AAAAAGTACTCTGCGTTGATACCACCCGTACTCTGCGTTGATACCTGTCTC16770.44053789230070056No Hit
AAAAAGTACTCTGCGTTGATACCACCCGTACTCTGCGTTGATACTGTCTCT15310.40218456357326926No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA13730.360678906457282No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCGTGTGCATCTCGTATGCCG13490.35437424968017006No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC12890.33861260773739005No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAGAGTACTTTTTCTGTCTCT12240.3215374956327117No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAGAGTACTTTTTCTGTCTC11740.3084027940137284No Hit
AAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACTGTCTCTTATA11250.2955307864271247No Hit
GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA11180.2936919282004671No Hit
AAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTTAT11080.2910649878766704No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCCTGTCTCTTATACACATCTC10290.27031215931867675No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC9970.26190595028252744No Hit
AAAAAGTACTCTGCGTTGATACCCGTACTCTGCGTTGATACCTGTCTCTTA9730.25560129350541544No Hit
TATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTCTGTCTCTTATA9540.2506101068902018No Hit
GAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTC8720.22906919623506913No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTCTGTCTCTTAT8220.2159344946160858No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCCTGTCTCT7560.19859668847902784No Hit
AAAAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGAGCC7280.19124125557239716No Hit
TTATACACATCTCCGAGCCCACGAGACTCGTGTGCATCTCGTATGCCGTCT6530.17153920314392218No Hit
GTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCC6490.17048842701440353No Hit
AAAAAGTACTCTGCGTTGATACCCGTACTCTGCGTTGATACTGTCTCTTAT6350.16681071056108818No Hit
GTATCAACGCAGAGTACGGGTATCAACGCAGAGTACTTTTTCTGTCTCTTA6350.16681071056108818No Hit
AAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCACGA6190.16260760604301353No Hit
GGTATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCT5770.15157445668306754No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACCTGTCTCTTATACACATCTCCG5180.1360755087726672No Hit
AAAGTACTCTGCGTTGATACCACCCGTACTCTGCGTTGATACTGTCTCTTA5120.13449934457838922No Hit
AAAAAGTACTCTGCGTTGATACCACCCGTACTCCTGTCTCTTATACACATC4970.1305589340926942No Hit
GGTATCAACGCAGAGTACTTTTTTTTCTGTCTCTTATACACATCTCCGAGC4890.12845738183365688No Hit
TATCAACGCAGAGTACGGGTATCAACGCAGAGTACTTTTTCTGTCTCTTAT4850.12740660570413823No Hit
AAAGTACTCTGCGTTGATACCACCCGTACTCTGCGTTGATACCTGTCTCTT4690.12320350118606356No Hit
AAAAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGA4470.1174242324737109No Hit
AAAAAGTACTCTGCGTTGATACCACCCGTACTCTGCGTTGATACCCTGTCT4430.11637345634419223No Hit
AAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGC4420.11611076231181257No Hit
ATACCACTGCTTCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCTC4330.11374651602039557No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAGAGTACTTTCTGTCTCTTA4320.1134838219880159No Hit
AAAAAGTACTCTGCGTTGATACCCCCGTACTCTGCGTTGATACTGTCTCTT4240.11138226972897856No Hit
ATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCT4240.11138226972897856No Hit
GAGTACGGGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATC4210.11059418763183958No Hit
GTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCG4130.10849263537280222No Hit
AAAAAGTACTCTGCGTTGATACCCCCGTACTCTGCGTTGATACCTGTCTCT4080.1071791652109039No Hit
AAAGTACTCTGCGTTGATACCCGTACTCTGCGTTGATACTGTCTCTTATAC4070.10691647117852424No Hit
AAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCACG4050.10639108311376491No Hit
GTATCAACGCAGAGTACGGGGGTATCAACGCAGAGTACTTTTTCTGTCTCT4030.10586569504900556No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATCTGTCTCTTAT3900.10245067262806991No Hit
AAAGTACTCTGCGTTGATACCCGTACTCTGCGTTGATACCTGTCTCTTATA3890.10218797859569025No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCTGTCTCTTATACACATCTC3880.10192528456331057No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC37900.043.4545645
GCCGTCT1250.037.79834745
CTCGTTT301.1387815E-437.4983628
TCGTTTG250.00210579535.99842529
TACCACG250.00210579535.99842520
TATTCGA250.00210579535.99842510
CCACGTA250.00210579535.99842522
ATGCCGT47400.034.74531644
TATGCCG48950.033.64510743
TCTCGTA49450.033.44140638
ATCTCGT49250.033.44016337
TCGTATG49550.033.41932340
GTATGCC49350.033.372442
CATCTCG49050.033.34716436
CTCGTAT49550.033.3285139
CGTATGC49900.033.27509741
TCGTGTG49300.033.22369829
GTGCATC48850.033.20734833
ACTCGTG49600.033.1588327
GACTCGT49950.033.151726
Produced by FastQC (version 0.11.2)