FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0120_pe_27.35100000046aaf.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0120_pe_27.35100000046aaf.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences235593
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGTGTGCATCTCGTATGCCGTC2614211.096254982108976No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCGTGTGCATCTCGTATGCCGT70923.010276196661191No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACTGTCTCTTA12540.5322738791050668No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTT12360.5246335841896831No Hit
TATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTTA11100.47115151978199693No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCGTGTGCATCTCGTATGCCG10720.4550220082939646No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTT9800.41597161205978106No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA9440.4006910222290136No Hit
GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA9190.39007950151320286No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC8940.37946798079739213No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC8730.37055430339611106No Hit
AAAAAGTACTCTGCGTTGATACCACCCGTACTCTGCGTTGATACTGTCTCT7870.33405067213372214No Hit
AAAAAGTACTCTGCGTTGATACCACCCGTACTCTGCGTTGATACCTGTCTC6060.25722326215125235No Hit
TTATACACATCTCCGAGCCCACGAGACTCGTGTGCATCTCGTATGCCGTCT5650.23982036817732275No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAGAGTACTTTTTCTGTCTCT5570.23642468154826332No Hit
AAAAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGAGCC5290.2245397783465553No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCCTGTCTCTTATACACATCTC4950.21010811017305267No Hit
AAAAAGTACTCTGCGTTGATACCCGTACTCTGCGTTGATACCTGTCTCTTA4910.20841026685852299No Hit
AAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACTGTCTCTTATA4800.20374119774356622No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAGAGTACTTTTTCTGTCTC4780.2028922760863014No Hit
AAAAAGTACTCTGCGTTGATACCCGTACTCTGCGTTGATACTGTCTCTTAT4580.19440305951365278No Hit
AAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGC4450.18888506874143118No Hit
AAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTTAT4380.1859138429410042No Hit
GTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCC4270.18124477382604748No Hit
GAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTC4220.1791224696828853No Hit
AAAAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGA4100.17402893973929615No Hit
GTATCAACGCAGAGTACGGGTATCAACGCAGAGTACTTTTTCTGTCTCTTA4070.17275555725339886No Hit
TATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTCTGTCTCTTATA4060.17233109642476643No Hit
GGTATCAACGCAGAGTACTTTTTTTTCTGTCTCTTATACACATCTCCGAGC4040.17148217476750158No Hit
TATCAACGCAGAGTACGGGTATCAACGCAGAGTACTTTTTCTGTCTCTTAT4030.17105771393886915No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTCTGTCTCTTATACACATCTCCGA3870.16426634068075027No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTCTGTCTCTTAT3520.14941021167861523No Hit
AAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCACGA3250.13794976930553965No Hit
AAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCC3120.13243177853331806No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCCTGTCTCT3020.12818717024699378No Hit
GTATCAACGCAGAGTACTTTCTGTCTCTTATACACATCTCCGAGCCCACGA2780.11800011035981546No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACCTGTCTCTTATACACATCTCCG2770.11757564953118302No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA2690.11417996290212357No Hit
AAAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAG2620.11120873710169657No Hit
AAAAAGTACTCTGCGTTGATACCCGTACTCCTGTCTCTTATACACATCTCC2450.10399290301494526No Hit
GGTATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCT2430.10314398135768041No Hit
AAAAAGTACTCTGCGTTGATACCACCCGTACTCCTGTCTCTTATACACATC2370.10059721638588583No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC27550.043.85662545
GCCGTCT700.038.5714345
CCACGTA250.002103716636.020
ATGCCGT34350.035.04366744
ATCTCGT36050.033.82801437
TCTCGTA36000.033.7538
CTCGTAT35950.033.73435239
GCATCTC36000.033.687535
CATCTCG36150.033.67219536
TATGCCG35850.033.64016743
GTGCATC35800.033.62430233
CGTATGC36300.033.59504341
TCGTATG36300.033.5330640
GTGTGCA36150.033.48547431
CGTGTGC36150.033.48547430
TCGTGTG36250.033.45517329
GTATGCC36250.033.45517342
GACTCGT36600.033.44262326
TGCATCT36350.033.42503434
ACTCGTG36350.033.42503427
Produced by FastQC (version 0.11.2)