FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0120_pe_25.35100000046a6b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0120_pe_25.35100000046a6b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences132694
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGTGTGCATCTCGTATGCCGTC1351710.186594721690506No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCGTGTGCATCTCGTATGCCGT18871.422068819991861No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCGTGTGCATCTCGTATGCCG4370.3293291331936636No Hit
TTATACACATCTCCGAGCCCACGAGACTCGTGTGCATCTCGTATGCCGTCT4050.30521349872639303No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTACAG206.999144E-445.0150835
TGCTTGG302.151577E-644.99811631
AGGTGTG207.0121646E-444.998117
TGCCGTC13200.042.782345
TTGCTTG356.2083836E-638.56981330
GGACTCT250.002100842535.998499
GTGGACT250.002100842535.9984911
CAATCCT250.002100842535.9984937
ATGCCGT16050.035.1854444
GCCCACG16500.034.36219818
AGCCCAC16500.034.36219817
CACGAGA16500.034.36219821
CCCACGA16500.034.36219819
GAGCCCA16500.034.36219816
CCACGAG16500.034.36219820
AGACTCG16400.034.29734425
GACTCGT16400.034.29734426
CCGAGCC16550.034.2583814
ACGAGAC16550.034.2583822
TCCGAGC16500.034.2258413
Produced by FastQC (version 0.11.2)