FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0120_pe_21.351000000469d6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0120_pe_21.351000000469d6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences317423
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTAACTGGATCTCGTATGCCGTC8704827.42334361404183No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTAACTGGATCTCGTATGCCGT124373.918115574485781No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTAACTGGATCTCGTATGCCG44601.405065165410194No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTT15420.48578710427410743No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTT14420.4542834010137891No Hit
AAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTTAT12050.37961962428683493No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACTGTCTCTTA10290.32417310654867476No Hit
AAAAAGTACTCTGCGTTGATACCACCCGTACTCTGCGTTGATACCTGTCTC9900.3118866622771507No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTCTGTCTCTTAT9490.2989701439404202No Hit
TATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTTA9460.29802503284261067No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAGAGTACTTTTTCTGTCTC8060.2539198482781651No Hit
AAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACTGTCTCTTATA7810.2460439224630855No Hit
TATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTCTGTCTCTTATA7350.23155221896333913No Hit
ATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCT7090.22336125611565638No Hit
GATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGA7070.22273118205045003No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCTGTCTCTTATACACATCT6790.21391014513756093No Hit
AAAAAGTACTCTGCGTTGATACCACCCGTACTCTGCGTTGATACTGTCTCT6260.19721318240959224No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAGAGTACTTTTTCTGTCTCT5790.18240644187724267No Hit
ATACCACTGCTTCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCTC5640.17768088638819493No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCCTGTCTCT5550.1748455530947663No Hit
GATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCG5290.16665459024708354No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCCTGTCTCTTATACACATCTC5240.16507940508406765No Hit
GGTATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCT5230.16476436805146444No Hit
GTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCGA5030.15846362739940079No Hit
AAAGTACTCTGCGTTGATACCACCCGTACTCTGCGTTGATACCTGTCTCTT5000.15751851630159125No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCG4940.15562829410597218No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCTGTCTCTTATACACATCTC4800.1512177756495276No Hit
GAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTC4740.14932755345390852No Hit
GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA4710.14838244235609896No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA4650.14649222016047986No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAGAGTACTTTCTGTCTCTT4470.14082155357362258No Hit
AAAAAGTACTCTGCGTTGATACCCGTACTCTGCGTTGATACCTGTCTCTTA4460.1405065165410194No Hit
TTATACACATCTCCGAGCCCACGAGACCTAACTGGATCTCGTATGCCGTCT4410.13893133137800348RNA PCR Primer, Index 23 (95% over 21bp)
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC4380.13798622028019394No Hit
TTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATAC4240.1335757018237494No Hit
GTATCAACGCAGAGTACGGGTATCAACGCAGAGTACTTTTTCTGTCTCTTA4090.12885014633470165No Hit
GATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATC3900.12286444271524119No Hit
AAAGTACTCTGCGTTGATACCACCCGTACTCTGCGTTGATACTGTCTCTTA3850.12128925755222526No Hit
GGTATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTCTGTCTCTTA3670.11561859096536797No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCCTGTCTCTTATACACATC3460.10900281328070115No Hit
AAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCCTGTCTCTTA3440.10837273921549478No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAACTGTCTCTTATACA3440.10837273921549478No Hit
TACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTC3420.10774266515028842No Hit
TTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACTGTCTCTTATACA3350.10553740592206613No Hit
GATACCACTGCTTCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCT3350.10553740592206613No Hit
AAAAAGTACTCTGCGTTGATACCCCCGTACTCTGCGTTGATACCTGTCTCT3350.10553740592206613No Hit
GATACCACCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCTCCGAG3270.10301710966124067No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC3230.10175696153082794No Hit
AAAGTACTCTGCGTTGATACCCGTACTCTGCGTTGATACCTGTCTCTTATA3230.10175696153082794No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCCTGTCTCTTATACACATCT3180.10018177636781204No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC90250.044.39855645
GCCGTCT1150.041.0840845
ATGCCGT101750.039.38053544
GGGAACT301.1385304E-437.49737518
GTATGCC107300.037.28070442
TATGCCG107500.037.2741343
TGGATCT106750.037.2409534
TCTCGTA107550.037.2358938
CTCGTAT107550.037.2358939
ATCTCGT107500.037.23227737
TCGTATG107750.037.22941240
GATCTCG107100.037.22428536
CTGGATC106750.037.19880333
GGATCTC107000.037.1959935
CGTATGC107800.037.19127341
ACTGGAT107000.037.17496532
CTAACTG107150.037.1439229
TAACTGG106850.037.1429330
AACTGGA106950.037.1292431
CCTAACT107450.037.06115728
Produced by FastQC (version 0.11.2)