FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0120_pe_2.3510000004677b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0120_pe_2.3510000004677b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10440
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGCTCTGGATCTCGTATGCCGTC261125.009578544061302No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGCTCTGGATCTCGTATGCCGT2041.9540229885057472No Hit
TTATACACATCTCCGAGCCCACGAGACAGCTCTGGATCTCGTATGCCGTCT690.6609195402298851No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGCTCTGGATCTCGTATGCCG590.5651340996168582No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC2800.043.39285745
CACGAGA3100.039.91935321
ACGAGAC3100.039.91935322
CGTATGC3100.039.91935341
TATGCCG3050.039.83606743
ATGCCGT3050.039.83606744
CGAGACA3050.039.83606723
CCACGAG3150.039.28571320
TCGTATG3150.039.28571340
CAGCTCT3100.039.19354628
CTCTGGA3100.039.19354631
GTATGCC3100.039.19354642
AGCTCTG3100.039.19354629
ATCTCGT3100.039.19354637
GACAGCT3100.039.19354626
TCTGGAT3100.039.19354632
GGATCTC3100.039.19354635
TCTCGTA3100.039.19354638
CTGGATC3100.039.19354633
TGGATCT3100.039.19354634
Produced by FastQC (version 0.11.2)