FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0120_pe_17.35100000046950.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0120_pe_17.35100000046950.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75319
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTAACTGGATCTCGTATGCCGTC804410.67990812411211No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTAACTGGATCTCGTATGCCGT10011.32901392742867No Hit
TTATACACATCTCCGAGCCCACGAGACCTAACTGGATCTCGTATGCCGTCT2780.3690967750501201RNA PCR Primer, Index 23 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACCTAACTGGATCTCGTATGCCG1970.26155418951393405No Hit
ATACACATCTCCGAGCCCACGAGACCTAACTGGATCTCGTATGCCGTCTTC1730.22968971972543448Illumina PCR Primer Index 2 (95% over 21bp)
GTTTTAGTTTTCAAACTTCTCAAAACAGAAAAGTTTTCTCTTGAACCTTGA980.1301132516363733No Hit
TGTCAGATAAATGCAAATGAAGTAACATTGAGATGCTACACCTTGTACACA950.12613019291281086No Hit
TTTTCATGCTCTTCTTCATTGGAAGAGTGATGCATATCAACCTAGTTACCA920.1221471341892484No Hit
ATTCATAAGATCAATAACCTCTTTTCGAGGTGATGCATCTTCAAATAAGAC860.1141810167421235No Hit
AATTAAGACTTCTGCTATTTGGAAAACACCATTAAAAGACAGAAATGCAAC860.1141810167421235No Hit
TCTCTTAATTGAGCTTCTTTTAAACTCGTTAACTCCACTCCTGAAACAGTT820.10887027177737356No Hit
ATTCAACAATATGAGTTTAAAGCACACATCACAAAGAAGTCTCTCAGAATG760.10090415433024869No Hit
AATTAGACCATTTTGTGAGTGTATTTACTACCTCTTGATCTGTATCCATGT760.10090415433024869No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTGAT206.971495E-445.0259171
TAGCTTG206.971495E-445.0259175
TGTCACA206.9943746E-444.99601730
TGCCGTC8650.040.5744445
TTGAGAT301.1259931E-437.5215954
ATGCCGT9550.036.7506744
GCCCACG9800.036.7314418
AGCCCAC9850.036.5449917
GAGCCCA9850.036.5449916
CGAGCCC9850.036.5449915
CCCACGA9800.036.5018719
CCGAGCC9800.036.5018714
CACGAGA9700.036.414321
CGAGACC9700.036.414323
CTCCGAG9850.036.3165812
TCCGAGC9850.036.3165813
CCACGAG9800.036.27229720
GAGACCT9650.036.136724
GGATCTC9800.036.04272535
TCTCGTA9800.036.04272538
Produced by FastQC (version 0.11.2)