FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0120_pe_13.351000000468e4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0120_pe_13.351000000468e4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences165601
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACGGCTGCATCTCGTATGCCGTC105826.390058031050537TruSeq Adapter, Index 11 (95% over 24bp)
TCTTATACACATCTCCGAGCCCACGAGACACGGCTGCATCTCGTATGCCGT12220.7379182492859343TruSeq Adapter, Index 11 (95% over 23bp)
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA6560.39613287359375854No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5630.3399737924287897No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5380.3248772652339056No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5160.3115923213024076No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4960.2995150995465003No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA4470.2699259062445275No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA4430.26751046189334604No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3600.2173899916063309No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3520.21255910290396796No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA3290.1986702978846746No Hit
GTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3180.19202782591892562No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA3110.18780079830435806No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3110.18780079830435806No Hit
TTATACACATCTCCGAGCCCACGAGACACGGCTGCATCTCGTATGCCGTCT3080.185989215040972TruSeq Adapter, Index 11 (96% over 25bp)
CTCTTATACACATCTCCGAGCCCACGAGACACGGCTGCATCTCGTATGCCG2840.17149654893388325RNA PCR Primer, Index 11 (95% over 22bp)
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA2650.16002318826577133No Hit
GTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2180.13164171713938927No Hit
GAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA2170.13103785605159388No Hit
GTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA2050.12379152299804953No Hit
GGTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA1890.1141297455933237No Hit
TATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAA1880.11352588450552835No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC1690.10205252383741643No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGTG207.0171803E-444.99818820
AGACTCG207.0171803E-444.99818825
GAGACTC207.0171803E-444.99818824
AAATTCG207.0171803E-444.99818814
CGTGTGC207.0171803E-444.99818830
CGAGACT207.0171803E-444.99818823
TGCCGTC11400.042.0377845
ATGCCGT13050.036.7226644
GTGCATC250.002102330635.99855433
TGGGTGC250.002102330635.99855421
GTGTGCA250.002102330635.99855431
AATTCGT250.002102330635.99855415
TATGCCG13450.035.79781743
CGTATGC13500.035.66523441
ACGGCTG13150.035.5879229
GTATGCC13550.035.53362342
CACGGCT13300.035.52488328
GCATCTC13250.035.4891435
ACGAGAC13700.035.30879622
GAGACAC13450.035.2959824
Produced by FastQC (version 0.11.2)