H23LNBCXX_l02n01_bir_0120_pe_11.35100000046894.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H23LNBCXX_l02n01_bir_0120_pe_11.35100000046894.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	120824
Sequences flagged as poor quality	0
Sequence length	51
%GC	36

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACACGGCTGCATCTCGTATGCCGTC	7838	6.487121763887969	TruSeq Adapter, Index 11 (95% over 24bp)
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA	1043	0.863239091571211	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	923	0.7639210752830563	No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	710	0.5876315963715818	No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	705	0.5834933456929087	No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA	696	0.5760444944712971	No Hit
TCTTATACACATCTCCGAGCCCACGAGACACGGCTGCATCTCGTATGCCGT	669	0.5536979408064623	TruSeq Adapter, Index 11 (95% over 23bp)
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA	623	0.5156260345626696	No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	588	0.48665827981195786	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	550	0.45520757465404227	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	534	0.44196517248228834	No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA	497	0.41134211746010724	No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	470	0.38899556379527245	No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	449	0.3716149109448454	No Hit
GTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	369	0.3054029000860756	No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA	359	0.2971263987287294	No Hit
GGTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA	301	0.2491226908561213	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	301	0.2491226908561213	No Hit
GTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	276	0.22843143746275574	No Hit
GAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA	260	0.21518903529100178	No Hit
GTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	253	0.20939548434085942	No Hit
TATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAA	220	0.1820830298616169	No Hit
ATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	213	0.17628947891147453	No Hit
GTATCAACGCAGAGTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAA	198	0.1638747268754552	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACGGCTGCATCTCGTATGCCG	193	0.15973647619678208	RNA PCR Primer, Index 11 (95% over 22bp)
GGTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAA	188	0.15559822551810898	No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA	179	0.14814937429649738	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTT	169	0.13987287293915118	No Hit
TTATACACATCTCCGAGCCCACGAGACACGGCTGCATCTCGTATGCCGTCT	166	0.1373899225319473	TruSeq Adapter, Index 11 (96% over 25bp)
GAGTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	159	0.13159637158180496	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	158	0.13076872144607032	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTT	141	0.11669866913858173	No Hit
TATCAACGCAGAGTACGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAA	135	0.111732768324174	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT	131	0.1084221677812355	No Hit
GTACGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA	128	0.10593921737403166	No Hit
ATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA	127	0.10511156723829702	No Hit
GAGTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA	123	0.10180096669535853	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTGACC	20	7.0082187E-4	45.0	8
TGCCGTC	830	0.0	42.56024	45
ATGCCGT	890	0.0	39.69101	44
TATGCCG	935	0.0	37.780746	43
GTATGCC	935	0.0	37.780746	42
CTCGTAT	930	0.0	37.741936	39
ACGGCTG	930	0.0	37.741936	29
TCTCGTA	930	0.0	37.741936	38
ACACGGC	940	0.0	37.57979	27
CGTATGC	940	0.0	37.57979	41
ACGAGAC	940	0.0	37.57979	22
ATCTCGT	935	0.0	37.540104	37
CACGGCT	935	0.0	37.540104	28
GCATCTC	935	0.0	37.540104	35
TGCATCT	930	0.0	37.5	34
CACGAGA	945	0.0	37.380955	21
AGACACG	945	0.0	37.380955	25
CGAGACA	945	0.0	37.380955	23
CATCTCG	940	0.0	37.340427	36
TCGTATG	940	0.0	37.340427	40

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content