FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0120_pe_1.35100000046752.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0120_pe_1.35100000046752.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11595
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGCTCTGGATCTCGTATGCCGTC258022.2509702457956No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGCTCTGGATCTCGTATGCCGT1591.371280724450194No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGCTCTGGATCTCGTATGCCG790.6813281586890901No Hit
TTATACACATCTCCGAGCCCACGAGACAGCTCTGGATCTCGTATGCCGTCT560.4829667960327727No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC2950.041.94915445
ATGCCGT3200.038.67187544
ATCTCCG3350.037.61194210
GCCCACG3350.037.61194218
TATGCCG3350.037.61194243
CACGAGA3350.037.61194221
CCCACGA3350.037.61194219
ACGAGAC3350.037.61194222
TCTCCGA3350.037.61194211
CGAGACA3350.037.61194223
AGACAGC3350.037.61194225
CAGCTCT3300.037.528
AGCTCTG3300.037.529
GTATGCC3400.037.05882342
ATCTCGT3400.037.05882337
CCACGAG3400.037.05882320
GAGCCCA3400.037.05882316
TCTCGTA3400.037.05882338
CGTATGC3400.037.05882341
CGAGCCC3400.037.05882315