FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0120_pd_96.35100000046738.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0120_pd_96.35100000046738.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences132
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTC53.787878787878788No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC32.272727272727273No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGC21.5151515151515151No Hit
ATGTAGTAAATGGATGAGCAGGATAACGTGTTTTTTCTTTACGATTCACAT10.7575757575757576No Hit
GGAAACTGTAACTTAGCATCTTTGATGATATATGTGACGTATGTTATCTTT10.7575757575757576No Hit
GTACAACCCCTGTTTTTGGTCCCAAGGCTCGCTTCGGCGGGTCGATCCGGG10.7575757575757576No Hit
GGTTGAGCCCGGGGATTTCACATCTGACTTAACAAACCGCCTGCGTGCGCT10.7575757575757576No Hit
GTATCAACCAGAGTACGGGGTGCGGACCACCACTGTCTCTTATACACATCC10.7575757575757576No Hit
GTTCTACATCTGTTATGCATGTATTTGGATGGAACATCTTCTGCTCTGCAC10.7575757575757576No Hit
CTTAGTCATCCGGCGGACGGGTTTCCCACCCGTCATTCGCTACTCATGCCT10.7575757575757576No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCC10.7575757575757576No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTT10.7575757575757576No Hit
GTGTAAGGGATGGAGCAGAAGATTTACCTTGACGTTTTTCAAATTCAACAA10.7575757575757576No Hit
ACTTCAGATCGCTGAACGAAAGCGGCGCCCCTTCCTGGTAGACGTTACCGA10.7575757575757576No Hit
GTGCCAGACCTGGCTCCATTCAAATCATGAGAGTGGAAGAAGTGGCTGCTA10.7575757575757576No Hit
TTGATGGACCATCAACAGTCTTCTGAGTAGCAGTGATTGAGCTGTCTCTTA10.7575757575757576No Hit
CGTTGATACCACCCGTACTCTGCGTTGATACTGTCTCTTATACACATCTCC10.7575757575757576No Hit
GTGTTTTGCTCTCTGTGTTTCTTGATTATTTTTTGTTTCCTTGAATTTATT10.7575757575757576No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTTA10.7575757575757576No Hit
ATTGTAAGGGTTAAAGTGGATAAAAAACCCCCTCACTATTTCGTATCTGTC10.7575757575757576No Hit
CTTATACACATCTCCGAGCCCACGAGACTCTGGATAATCTCGTATGCCGTC10.7575757575757576No Hit
CTTATACACACTCCGAGCCCAGAGACGACGCTATATCTCGTATGCCGTCTT10.7575757575757576Illumina PCR Primer Index 11 (95% over 22bp)
CTATCGGTCATTGAGGAATACTTAGGCTTAGAGGGTGGTCCCCCTTTTTCG10.7575757575757576No Hit
GCTCAGATGAGCTGGAGTGGCTGGTTCAACATCGGGGCGCCGGCGGGTGGC10.7575757575757576No Hit
GGATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTATGAAGA10.7575757575757576No Hit
TTTTTTTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCT10.7575757575757576No Hit
GGAGAGGTGTCCAGTGGCTCCGGGGAGACGTTCACCTACCCTGACTTTCTC10.7575757575757576No Hit
ATCCCAAGTCAATCCTCTGATGTACACAATCGCCAATATAATTGTTATCCC10.7575757575757576No Hit
CTTATACACATCTCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTCC10.7575757575757576No Hit
GGTATCAACGCAGAGTACGGGGTATCAACGCAGACTACTCTGTCTCTTATA10.7575757575757576No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTGCACCATCTCGTATGCCGTC10.7575757575757576No Hit
GAACGACGCTCTCCCATTCCCAGAACCACCATTGCCCATTTTCTTGCCACC10.7575757575757576No Hit
CGTTGATACCACCCGTACTCTGCGTTGATACCTGTCTCGTATACACATCTC10.7575757575757576No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGTGTGTATCTCGTATGCCGTC10.7575757575757576No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACC10.7575757575757576No Hit
CTACGCATTTCACCGCTACACCTGGACTTCTACCCCCCTCTACGAGACTCA10.7575757575757576No Hit
CCCTTCTGGTATGGGCGATCATGAGCAAGCACCGCCCAAATTGTCTGCGCG10.7575757575757576No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTTTT10.7575757575757576No Hit
GGTGTCACCTCCCCCATGTATGGCATGTAATCTACTTCATGATTTCCTGCA10.7575757575757576No Hit
TCACACACAAGTGCATCATAGAAACGAAAACAAAAAGGGAAATGAAACAAA10.7575757575757576No Hit
GGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAGGACTCTGCGTTGATACT10.7575757575757576No Hit
TCTTTGTGGTGAGAAAACTCAAAATCCTCTCTTCACTCTGTCTTAAGATAC10.7575757575757576No Hit
AGCTTTTTTGATCACGATCCAGGTGACCCCTAAGCATGGAGGGCCTGGAAG10.7575757575757576No Hit
GGGTGTTGGTCGATTAAGACAGCAGGACGGCTGTATCTTATACACATCTCC10.7575757575757576No Hit
TTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTCT10.7575757575757576Illumina Paired End PCR Primer 2 (95% over 21bp)
CCATTTGTGTTAGTGGGAACTTATCTGTAGTTGAACTGTCTCTTATACACA10.7575757575757576No Hit
GTCTTGGGACCCCGAACTTCGGGGGCTGTTTTTTTGGGCGCGGCTGTGCCC10.7575757575757576No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT10.7575757575757576No Hit
GGGTTCCTTAGGGACCGATTCACTCTGCGTAGATTGACTGAACGCAGAAAG10.7575757575757576No Hit
GGGAAACAACCCAGACCGCCAGCTAAGGTCCCAACGTCATGGTTAAGTGGG10.7575757575757576No Hit
GGTGTCGGCCTCGAAAATAATCGTGTACCAGCGCAGGCGCCAGCCGGCCAG10.7575757575757576No Hit
GTCGTTGGCTTCCACGGTGAACTCGCCATTGGCCGCCAGCTTGTGCAGCCA10.7575757575757576No Hit
GTATTGGAGCGGTCTCGTTTGTGTACGACAAAGCAGGTCCTGTCTCTTATA10.7575757575757576No Hit
CTGCTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTGATA10.7575757575757576No Hit
AATAAGGTCTTTCTCAGAGAAGCAAAAACTGAAGAAATTCATCACTACCAC10.7575757575757576No Hit
CGACGAGACAGATGATCCCGACGGTGTTGGAGATACCATTGGCTTTAGTAA10.7575757575757576No Hit
CACCAGCATCGGTAAGCTCTGCAAGCATTTCCAGTCCTATCACCAGTCCGT10.7575757575757576No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCTGGATAATCCCGTATGCCGT10.7575757575757576No Hit
GTGGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAA10.7575757575757576No Hit
GTGTAACAACTCACCTGCCGAATCAACTAGCCCCGAAAATGGATGGCGCTT10.7575757575757576No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCG10.7575757575757576No Hit
AGACCGAGGCCCGCGCATGACACCCCATCTGCGCGGCGCCTGTGCGATCGT10.7575757575757576No Hit
GCAGAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATC10.7575757575757576No Hit
GTACTTGTTCGCTATCGGTCTCTCGCCAATATTTAGCTTTAGATGGAATTT10.7575757575757576No Hit
CTCCTAAAGAGTAACGGAGGTGTGCGAAGGTAGGCTCAAGCTAAGATTCTG10.7575757575757576No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTGATACCA10.7575757575757576No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTGTTTTTATCCCCCCCGTTCTCCGTT10.7575757575757576No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTATACCC10.7575757575757576No Hit
CCCAAGGCGCGTGCGCAAGTGCTGCACACGCTGGGTTTTGGCCTGTCTCTT10.7575757575757576No Hit
GAGTACGGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGC10.7575757575757576No Hit
GATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCT10.7575757575757576No Hit
GTTCAACGCAGAGTACGGGATCCGCCCGCCTCGGCCTCCCAAAGTGCTGGG10.7575757575757576No Hit
TATCAACGCAGCGTACTTTTTTTTTTTGTTTTTTTTTTTTTTCTTTTCTTG10.7575757575757576No Hit
GTGTGTTCAAGTGGGATTGTTAAGAATAGTTCCACGAAACTGTCTCTTATA10.7575757575757576No Hit
AGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGG10.7575757575757576No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.7575757575757576No Hit
ACTTAGGAGGGTAAACTGGTGGGAGAACAGGAGGTTTGATCGGTGGGAGAG10.7575757575757576No Hit
TAAATGGCTAGACATACAATACTCATATTTTCCAAATCATCTACTAACTGT10.7575757575757576No Hit
CCTTTCTTCAGTAGCATCGTTGAGATTCTGAGCGTTTGAGCCGCTGTCTCT10.7575757575757576No Hit
GTATCAACGCAGAGTACTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCTT10.7575757575757576No Hit
CTTATACACATCTCCGAGCCCACGAGACTCTCGGTAATCTCGTATGCCGTC10.7575757575757576No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGC10.7575757575757576No Hit
CGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAG10.7575757575757576No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAG10.7575757575757576No Hit
CTCCATTTAAATATCACTACTTCTTTTTCAACAACAACCTTACGAATAAAG10.7575757575757576No Hit
ACTGCAAGCTTGCAAGTACGCGGGCGTCAACAACGTCCAAGGCGCTAAACC10.7575757575757576No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAA10.7575757575757576No Hit
AGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCACCGCAGAGTACGG10.7575757575757576No Hit
GAATAGGGCACAAGCAGTGAGCATGAAGACGATGTAGAACCTGTCTCTTAT10.7575757575757576No Hit
CTTATACACATCTCCGAGCCCACGAGACTCTGGACAATCTCGTATGCCGTC10.7575757575757576No Hit
GCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGACTGTCTC10.7575757575757576No Hit
GTTTATGGAATTCACCAATGTTTTTGATGTAGCTTCAGCCTATTCCTGTCT10.7575757575757576No Hit
TCTCTACCGATCTCTGTCTATCTCTCTTGCGAAAAAAAGTACTGTTACTGT10.7575757575757576No Hit
GCTCGCTACAAAGCATTGAAAGCTACATTATCATACTTATCTCTGTCTCTT10.7575757575757576No Hit
GTACGGGGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAG10.7575757575757576No Hit
ACTTTCAACGAGCCATGGGTCTTCGCTCATGCAGGTTACGACGTAGGCAAG10.7575757575757576No Hit
CAGCGATACTGACGTGCAAATCGTTCGTCTGACTTGGGTATAGGGGCGAAA10.7575757575757576No Hit
GCATCCGCGTGCTCCGGGCGTCGGCCTGTGGGCTCCCCATTCGACCCGTCT10.7575757575757576No Hit
TCTTTAACGGGTTTAGCGCCTTGGACGTTGTTGACGCCCGCGTACTTGCAA10.7575757575757576No Hit
GCTCCAAGTGGGCCATTTTTGGTAAGCAGAACTGGCGATGCGGGATGAACC10.7575757575757576No Hit
ATCGGAGCCTTATCAAACACATTCATCAGTGTCCGATGCCTTGCCAGTTGT10.7575757575757576No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAGTCCTCTGCG10.7575757575757576No Hit
TCAAGCGAGTTCGTCGGAGACAAGGTTGCCTATGCACTCTGTCTCTTATAC10.7575757575757576No Hit
TATCTGACGATAGCAAGTTATGTTTTGAGTTACTCCTGTCTCTAATACACA10.7575757575757576No Hit
CATATACACCATGGAATACTATGCAGCCATAAAAAACTGTCTCTTATACAC10.7575757575757576No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAAATATTCCCCCCCCTC10.7575757575757576No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTGTCCCCCCTC10.7575757575757576No Hit
CAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGCTGTCTCTTATA10.7575757575757576No Hit
GTACTTTTTTTTTTCTTTTTTTTTTTCAAAAAAAAAAAAAAAAAAAAAAAA10.7575757575757576No Hit
CCACTGCTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTG10.7575757575757576No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA10.7575757575757576No Hit
GTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATG10.7575757575757576No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAA10.7575757575757576No Hit
CAATAAGAAATTTCATCATTGAACACATCCTGTCTCTTATACACATCTCCG10.7575757575757576No Hit
GTCGATTGCTTGCGCGATGCCAACATCGTCCCTGGCATCAAAGTTGACAAG10.7575757575757576No Hit
ACATTCGAGGCACCATCGAACCCCAGAGAAGCAGTCAAGGAAGAAATCTGT10.7575757575757576No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAAC10.7575757575757576No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACC10.7575757575757576No Hit
GTACGGGAAGCAGTGGTATCAAGCAGAAAAAAAAAAAAAAAAAAAAAAAAA10.7575757575757576No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTGCTTTTAAACCCCCCGTACTTTG10.7575757575757576No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATCCAGT10.7575757575757576No Hit
GTACTTTTTTTTTTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTACT10.7575757575757576No Hit
GAGTACGGGTCGATGCAACGCGAAGAACCTTACCTGGTCTTGACATCCACG10.7575757575757576No Hit
GTTCATATGAAGGTGCTTTCTTTTGCATATATAGTTGTTTAATTCAATGTT10.7575757575757576No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTGCGTTTAAACCACTGAAAAAAAA10.7575757575757576No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers