FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0120_pd_93.351000000466d9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0120_pd_93.351000000466d9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50344
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTC20374.046162402669633No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGT2330.4628158271094867No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC2150.42706181471476246No Hit
TTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTCT1280.2542507548069283No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCA1000.19863340219291276No Hit
ATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTCTTC960.19068806610519623Illumina PCR Primer Index 7 (95% over 22bp)
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGC860.17082472588590497No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA820.16287938979818847No Hit
CATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAAT790.15692038773240108No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA730.14500238360082632No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAG640.12712537740346416No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA610.12116637533767678No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA590.11719370729381853No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA530.10527570316224376No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTAAA206.969075E-445.0000041
TGCCGTC3200.040.78125445
ATGCCGT3500.036.64285744
CCCACGA3650.036.3698619
GGATAAT3600.036.2532
TGGATAA3500.036.031
AGCCCAC3700.035.8783817
ATCTCGT3700.035.8783837
GCCCACG3700.035.8783818
CCACGAG3700.035.8783820
AATCTCG3650.035.7534236
CACGAGA3650.035.7534221
CGTATGC3650.035.7534241
CTCGTAT3650.035.7534239
CTGGATA3550.035.49295830
GACCCTG3500.035.35714326
GTATGCC3700.035.2702742
TATGCCG3700.035.2702743
TAATCTC3700.035.2702735
TCGTATG3700.035.2702740
Produced by FastQC (version 0.11.2)