FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0120_pd_92.351000000466bf.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0120_pd_92.351000000466bf.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences219396
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTC2446511.151069299349121No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGT54622.489562252730223No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCG15860.7228937628762603No Hit
TTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTCT7780.3546099290780142No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC25800.042.64275445
GCCGTCT1100.040.90660545
ATGCCGT31500.034.6407544
CCCTGGA33800.032.8160528
ACGAGAC33750.032.79800822
AATCTCG33500.032.77412836
CCCACGA33850.032.7675819
ATCTCGT33650.032.76175337
GCCCACG33950.032.7373318
GAGACCC33750.032.73134224
ACCCTGG33900.032.7192527
CTCGTAT33600.032.67658639
CCACGAG33950.032.67106220
TCTCGTA33550.032.65822238
CACGAGA33900.032.6528821
CGAGACC33850.032.63464723
CGTATGC33650.032.62803341
TATGCCG33450.032.62133443
AGCCCAC34100.032.59332717
AGACCCT33900.032.58651725