Basic Statistics
Measure | Value |
---|---|
Filename | H23LNBCXX_l02n01_bir_0120_pd_89.3510000004666f.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 25442 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 50 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTC | 7033 | 27.643267038754814 | No Hit |
TCTTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGT | 721 | 2.8338967062337868 | No Hit |
TTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTCT | 275 | 1.080889867148809 | No Hit |
CTCTTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCG | 208 | 0.8175457904252811 | No Hit |
ATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTCTTC | 68 | 0.2672745853313419 | Illumina PCR Primer Index 7 (95% over 22bp) |
TATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTCTT | 50 | 0.19652543039069256 | Illumina PCR Primer Index 7 (95% over 21bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TGCCGTC | 810 | 0.0 | 41.666668 | 45 |
ATGCCGT | 885 | 0.0 | 38.135593 | 44 |
AGCCCAC | 905 | 0.0 | 37.29282 | 17 |
GCCCACG | 905 | 0.0 | 37.29282 | 18 |
GAGCCCA | 905 | 0.0 | 37.29282 | 16 |
CGAGCCC | 905 | 0.0 | 37.29282 | 15 |
CCACGAG | 910 | 0.0 | 37.08791 | 20 |
TATGCCG | 910 | 0.0 | 37.08791 | 43 |
CACGAGA | 910 | 0.0 | 37.08791 | 21 |
CCCACGA | 910 | 0.0 | 37.08791 | 19 |
TCTCGTA | 910 | 0.0 | 37.08791 | 38 |
ACGAGAC | 910 | 0.0 | 37.08791 | 22 |
TCGTATG | 910 | 0.0 | 37.08791 | 40 |
TCCGAGC | 910 | 0.0 | 37.08791 | 13 |
CTCGTAT | 910 | 0.0 | 37.08791 | 39 |
AATCTCG | 905 | 0.0 | 37.0442 | 36 |
ATCTCGT | 905 | 0.0 | 37.0442 | 37 |
GGATAAT | 905 | 0.0 | 37.0442 | 32 |
CTGGATA | 900 | 0.0 | 37.0 | 30 |
CCTGGAT | 895 | 0.0 | 36.955307 | 29 |