FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0120_pd_89.3510000004666f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0120_pd_89.3510000004666f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25442
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTC703327.643267038754814No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGT7212.8338967062337868No Hit
TTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTCT2751.080889867148809No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCG2080.8175457904252811No Hit
ATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTCTTC680.2672745853313419Illumina PCR Primer Index 7 (95% over 22bp)
TATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTCTT500.19652543039069256Illumina PCR Primer Index 7 (95% over 21bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC8100.041.66666845
ATGCCGT8850.038.13559344
AGCCCAC9050.037.2928217
GCCCACG9050.037.2928218
GAGCCCA9050.037.2928216
CGAGCCC9050.037.2928215
CCACGAG9100.037.0879120
TATGCCG9100.037.0879143
CACGAGA9100.037.0879121
CCCACGA9100.037.0879119
TCTCGTA9100.037.0879138
ACGAGAC9100.037.0879122
TCGTATG9100.037.0879140
TCCGAGC9100.037.0879113
CTCGTAT9100.037.0879139
AATCTCG9050.037.044236
ATCTCGT9050.037.044237
GGATAAT9050.037.044232
CTGGATA9000.037.030
CCTGGAT8950.036.95530729