FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0120_pd_88.35100000046646.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0120_pd_88.35100000046646.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences326967
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC211416.465790125609007No Hit
TTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTCT33191.0150871494676834RNA PCR Primer, Index 44 (95% over 22bp)
TCTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGT13110.40095789483342353No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC6730.2058311695064028No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC5650.17280031318145256No Hit
ATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTCTTC5310.16240171026433858RNA PCR Primer, Index 44 (100% over 23bp)
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCC4130.1263124413167078No Hit
TATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTCTT4040.12355986995629528RNA PCR Primer, Index 44 (100% over 22bp)
CACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCT4010.12264234616949111No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTC3970.12141898112041888No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTTT3840.11744304471093413No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCG3690.11285542577691325No Hit
GTATCAACGCAGAGTACGGGCAGCTGTCTCTTATACACATCTCCGAGCCCA3490.10673860053155211No Hit
CATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAAT3460.10582107674474794No Hit
GTATCAACGCAGAGTACGGGTGGCTGTCTCTTATACACATCTCCGAGCCCA3430.10490355295794376No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAG3380.10337434664660348No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCA3270.10001009276165486No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC27350.036.11345745
CATATAG250.002100662836.0148123
GCCGTCT5200.034.61373545
ATGCCGT29400.033.3657644
TATCTCG29600.033.21632436
CTCGTAT29550.033.19639239
TATGCCG29500.033.17638843
ATCTCGT29750.033.12447737
GAGACAA29900.033.03354324
CGTATGC29700.033.02873241
ACATAAT29650.033.0085530
AGACAAC29950.032.97839725
ATAATAT29700.032.95297632
TCGTATG29850.032.93813340
TCTCGTA30000.032.92343538
CAACATA29750.032.89759428
GACAACA30000.032.8484426
TAATATC29800.032.842433
GTATGCC29800.032.842442
ACAACAT29950.032.82815627
Produced by FastQC (version 0.11.2)