FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0120_pd_86.351000000465f4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0120_pd_86.351000000465f4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences293721
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC189376.447274794788252No Hit
TCTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGT72772.4775211850701857No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACCTGTCTCT15060.5127314696599834No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCG8910.30334909659166354No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTT7840.26691996826920783No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC7080.24104507338596834No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACCTGTCT6200.21108466878432255No Hit
CACTATCATTAACTGAATCCATAGGTTAATGACTGTCTCTTATACACATCT5680.19338079333789548No Hit
TCATTAACCTATGGATTCAGTTAATGATAGCTGTCTCTTATACACATCTCC5650.19235941590829395No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGCTGTCTCTTATACACATCT5650.19235941590829395No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACA5270.17942196846667416No Hit
TTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTCT4800.16342038873624973RNA PCR Primer, Index 44 (95% over 22bp)
CTATCATTAACTGAATCCATAGGTTAATGACTGTCTCTTATACACATCTCC4680.15933487901784346No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACCTGTCTCTTA4460.151844777867432No Hit
AAAAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGA4330.14741880900582524No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC4120.14026916699861433No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA4000.1361836572802081No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACCTGTCTCTT3870.13175768841860133No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTC3640.12392712812498935No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA3460.11779886354737999No Hit
AAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGC3330.11337289468577323No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA3080.10486141610576023No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCC3050.10384003867615867No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC22200.042.7675245
GTTACCG250.002105225335.99768411
GCTTCGC250.002105225335.99768441
GCCGTCT1100.034.77049345
TCACGCA352.8079306E-432.1572154
CGACTTG352.816363E-432.1407920
ATGCCGT30100.031.54282244
CACCAAG801.0004442E-1030.935517
TATGCCG31050.030.57774243
ATCTCGT31050.030.43282537
CGTATGC31200.030.43073541
CGAGACA31400.030.38021323
TCGTATG31300.030.33351340
TATCTCG31100.030.31155436
CTCGTAT31200.030.28651439
CACGAGA31500.030.28376821
CCACGAG31550.030.23577320
TCTCGTA31550.030.02184138
GTACAGG901.2732926E-1129.99807213
CGTTCGA300.0051427129.99807216
Produced by FastQC (version 0.11.2)