FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0120_pd_82.3510000004657d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0120_pd_82.3510000004657d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences275910
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC6465823.434453263745425No Hit
TCTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGT272149.863361240984378No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCG56102.033271719038817No Hit
TTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTCT16380.5936718495161465RNA PCR Primer, Index 44 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGATT253.8839993E-544.9981888
ACATCCG207.025352E-444.9981887
TGCCGTC71900.043.2145345
GCCGTCT2950.038.8967445
ATTAAGG301.1355177E-437.5120853
ATGCCGT99700.031.14217644
CTATAAC300.005132463830.0096683
AAACACG300.005132463830.0096681
CTACACC300.005132463830.0096684
GAGGAAC300.00514157229.9987927
CAACTAG300.00514157229.9987944
CATCCGA300.00514157229.998798
TCTCGTA106850.029.14248738
TATGCCG106600.029.12640843
TATCTCG107000.029.10163336
ATCTCGT107100.029.09546737
GTATGCC106750.029.0854842
CGTATGC107150.029.08189241
TCGTATG107150.029.08189240
CTCGTAT107100.029.0744639
Produced by FastQC (version 0.11.2)