FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0120_pd_81.35100000046554.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0120_pd_81.35100000046554.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences126517
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC2940923.24509749677909No Hit
TCTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGT51454.066647169945541No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCG14081.1128939193942315No Hit
TTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTCT7560.5975481555838347RNA PCR Primer, Index 44 (95% over 22bp)
ATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTCTTC3680.290870001659856RNA PCR Primer, Index 44 (100% over 23bp)
TATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTCTT1800.14227337037710347RNA PCR Primer, Index 44 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGATA207.0094777E-445.036
TGCCGTC31550.042.43264445
AAACTCG301.1341885E-437.50000423
ATGCCGT37100.036.02425844
AACGTCG250.00210004336.034
GGAATCC250.00210004336.08
CATGCTA250.00210004336.029
CTAAGTA250.00210004336.030
ATGTCTT250.00210004336.035
CGCTCCC250.00210004336.021
CTTATAA250.00210004336.01
GTTAAAC250.00210004336.020
TTAAGGT250.00210004336.03
AGCATCA250.00210004336.042
ACTCGTA250.00210004336.025
ACACTAA250.00210004336.027
AACTCGT250.00210004336.024
CTCAGAC250.00210004336.07
GAAGGGT250.00210004336.013
CACGAGA39450.034.10646421
Produced by FastQC (version 0.11.2)