FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0120_pd_77.351000000464e8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0120_pd_77.351000000464e8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28727
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC302310.523201169631358No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGT7002.436731994291085No Hit
TTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTCT3021.0512758032512968Illumina PCR Primer Index 8 (95% over 21bp)
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC1630.5674104500992098No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACA1230.42816862185400495No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGC840.2924078393149302No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTT740.257597382253629No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCG660.22974901660458802No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCA580.20190065095554705No Hit
ATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTCTTC560.19493855954328682Illumina PCR Primer Index 8 (95% over 23bp)
GGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAA450.15664705677585547No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA430.1496849653635952No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCAC370.1287986911268145No Hit
GTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGG350.12183659971455425No Hit
TATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTCTT330.11487450830229401Illumina PCR Primer Index 8 (95% over 22bp)
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC320.1113934625961639No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACC300.10443137118390364No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAAC290.10095032547777352No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCG290.10095032547777352No Hit
GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA290.10095032547777352No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC4350.040.86207245
GAGGCGA250.002073133636.027
ACCGGGG250.002073133636.034
AACCGGG250.002073133636.033
CGGGGGA250.002073133636.036
GCGAACC250.002073133636.030
GGCGAAC250.002073133636.029
GAACCGG250.002073133636.032
CGAACCG250.002073133636.031
CCGGGGG250.002073133636.035
AATGAGG250.002073133636.024
ATGAGGC250.002073133636.025
AGACTTG5050.035.19801725
GAGACTT5050.035.19801724
GCCCACG5150.034.95145418
CCACGAG5150.034.95145420
CACGAGA5150.034.95145421
CCCACGA5150.034.95145419
ACGAGAC5150.034.95145422
ACTTGCT5100.034.8529427
Produced by FastQC (version 0.11.2)