FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0120_pd_75.351000000464a5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0120_pd_75.351000000464a5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences136276
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC4178530.662038803604453No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGT108717.9771933429217174No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCG18241.3384601837447532No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACA7410.543749449646306No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCC4580.33608265578678564No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTT4010.2942557750447621No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAGT3870.2839825060905809No Hit
TTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTCT3580.26270216325691975Illumina PCR Primer Index 8 (95% over 21bp)
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT3200.23481757609557075No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAA2920.2142710381872083No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCA2680.1966597199800405No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACC2540.18638645102585927No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTCGACACACT2460.18051601162347No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGT2350.1724441574451848No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATT2020.14822859491032903No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGA1970.14455957028383573No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACCTGTCTC1830.13428630132965452No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACTTTTT1820.13355249640435585No Hit
GTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACTGTCTC1820.13355249640435585No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACT1800.13208488655375855No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTTT1780.13061727670316123No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCAT1720.12621444715136929No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCTGTCTCTTATACACATCT1690.1240130323754733No Hit
GATACCACTGCTTCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCT1580.11594117819718806No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTC1560.11447356834659074No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCTGTCTCTTATACACATCTC1550.11373976342129208No Hit
ATACCACTGCTTCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCTC1520.11153834864539611No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCCTGTCTCTTATACACATC1510.11080454372009745No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTT1490.10933693386950014No Hit
ATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCT1480.10860312894420147No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGG1470.10786932401890281No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTCT1450.1064017141683055No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCCTGTCTCTTATACACATCT1430.10493410431770818No Hit
GATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATC1370.10053127476591622No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCATT351.2014061E-745.0000049
GTGTACG406.7393557E-945.019
ACGTATA207.011392E-445.029
ATTGTGC207.011392E-445.037
TGTACGG406.7393557E-945.020
TGTGTAC406.7393557E-945.018
TGCCGTC44500.043.9887645
TGCATTT451.9070285E-840.010
GCTGTCA403.4283948E-739.37528
TATGTGC301.1346491E-437.4999966
ACACGAA250.002100610636.017
GTGCAAA250.002100610636.011
AAACACG250.002100610636.015
CACGAAA250.002100610636.018
AACCATG250.002100610636.07
CGTATAC250.002100610636.030
CGAAAGT250.002100610636.020
TTGTGTA504.8252332E-836.017
GTGGACG250.002100610636.025
ACGAAAG250.002100610636.019
Produced by FastQC (version 0.11.2)