FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0120_pd_73.35100000046455.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0120_pd_73.35100000046455.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences131655
Sequences flagged as poor quality0
Sequence length51
%GC36

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC123389.371463294215943No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGT12560.9540085830390035No Hit
GATAGGCATCTATTTAGTTACTTATGTCAAATGAATAGCCAGTTGTTCTCA4050.30762219437165317No Hit
TTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTCT3490.26508677984125173Illumina PCR Primer Index 8 (95% over 21bp)
GTTGATACAGCAAAAATTATTGAAAAGACATTCCTTTTCCCTGAATTTCCT3160.2400212677072652No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCG3030.230146975048422No Hit
GAGTGTTTCAAAACTACTCAATCAAAAGAAAAGGTCAACTCTTTGAGATGA2840.2157153165470358No Hit
ATGTTTAAGGCATCAGAATTTAGTTTAATTGCCTGAAGGAAGGAAAATTAA2810.21343663362576432No Hit
ATTCTCTACAGCAGTGTATTAATAAGCACTATGCTTCAATGAATTGTGGTG2750.2088792677832213No Hit
GATTTACAAAGAGTGTATTTCCAAACTGCTCGATTAAAGAAAGGTTAAACT2730.20736014583570697No Hit
ATCATCAGGAAAGCATTTTGAAACATAGGTTATATCCATTGTCATCAATGT2680.2035623409669211No Hit
ATTCCGGAATAGATACGGAAAAGATGATTGAGAAGAAACTAAAATTTGCTG2600.19748585317686376No Hit
ATGTTGGAAAAGGAAATATCTTCACATAAAAACCAGACAGAAGCATTCTCA2460.18685199954426343No Hit
ATTCCATTCCATTTGAAGATGATTCCATTCAAGACCATTCGATGATTGCAT2370.1800159507804489No Hit
GGTGACTACAGTCTAGAATACTGTATTGTTACTTGAAGTTTGCTAAGAGAA2230.16938209714784855No Hit
ATACTTACCAAGAAAATAGCAAAAGAAAGCAGGGATTGCAAATCCTAGTCT2140.16254604838403403No Hit
ATAAACATTGGCATTATCTGTTTCAACTGACAGGAATTAATACTGAGTCAT2070.15722912156773386No Hit
GAATAGTCTCTTGGATGAATGGCTCTAGGAAAATTGTATATCCACATGCAA1940.14735482890889065No Hit
ATTAGTTTAACCCTTTTGAAGTTTGTTTTTGTTGAAGATGCAGAGAGACAC1920.14583570696137632No Hit
TTACAACATCTACATCAACATTGTCTTTGTCCCAATATTCATCGTTTTTCT1900.144316585013862No Hit
GAATTATGATCAAATGGAATCGAATGTAATCATCATCAAATGGAATCAAAA1880.14279746306634766No Hit
ATAGTATTTTGTGGGACAAAGACTGGTAATTTCACAAATTGAATAAAGAAG1860.1412783411188333No Hit
ATTCTTTTGTGGCTAAATTCAATGAATCTTGTGAAAATTTGTTGACTGAAA1850.14051878014507616No Hit
TACTAGTTATGTCCTATTCTCTTCCTCATTTTGCTAGTGGAGAATACTAAT1850.14051878014507616No Hit
ATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTCTTC1840.13975921917131898Illumina PCR Primer Index 8 (95% over 23bp)
GTACAGTGTTCTGTAAAAATTTGGAATTTGTTGCTAACATTTTAAAACCAG1840.13975921917131898No Hit
GCTAGAGTATTTAAGTTACTTGCTAATTCAGCAAAGAATGGATTCGATAGA1790.1359614143025331No Hit
ATATATTGGTGTGTGATTTTCTTTTCTTGTGTGATGTATTTGTCTGGCTTT1780.13520185332877596No Hit
TAGTTTCAGTTATGCAGGATGAATAAGCTTCTGAGGGAAAATATACATGGT1780.13520185332877596No Hit
TCCTTTAAGATGCACAAAAACAATTTTCTTCTAGAACAACATACCTGCCAT1760.13368273138126163No Hit
GTACAAAGTTTTAGAGACAGGCCAGATATATCTGCATTAGCCTTGAGAAAA1720.13064448748623297No Hit
GAAAATATTTGATTCAATCAAACAGCATTACTTGGTATGGTGTAGAGAAAA1710.12988492651247577No Hit
TCTAACAACTGCACATGATTCTAACAAACTGATTATATGGCTTTGAAATGC1690.12836580456496147No Hit
ATATACAAGTTGCAGGAAAACATCTCTATTACACCCTAATTACTACATATC1660.12608712164368993No Hit
TTTAACTAAGTACTGAAATCCTGCAAAAAAGAATAGCTTTTGGTATTTTCC1650.12532756066993278No Hit
TGAATAAACAAAGAACATGGATTCTTCAGATGGGATCTACTCTTGGTGAAG1640.12456799969617562No Hit
ATCTATGGTGATATCTACTCTATCATTCCTGATGTTGCTAGTTTGTTTTTC1620.12304887774866127No Hit
TCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCT1620.12304887774866127No Hit
ATATAAACCTTGTTGTACCATCTTCACTGCTTTTTTAAAGTTACCATTCCA1590.12077019482738977No Hit
TATTTAAATGGATTCAGTGCTTACCATCATATCTTTTCCATGGCTTTTCTA1590.12077019482738977No Hit
ATATAATTCAACACAACACAACACAACACAACATGACTTAATGCACTACAC1560.11849151190611827No Hit
GTATAGTGTTTCAGTAGTCCTGATACATTTTACTGAAGATTTGATCTTGGT1550.11773195093236109No Hit
ATCAAAAGAATCCAGCTAACCTAACTTGAAAAAAATCCCAATTCAAAAATT1550.11773195093236109No Hit
TGTTTATGCATTGATTTTGTACCCTGAAACTTTGCTGAAGTCATTTATCAG1530.11621282898484676No Hit
AGACTATTCACACTGATGAATACTTTATTGAGTACACATTTCAACTTTGAC1530.11621282898484676No Hit
AGCATTAGTCACAATAGTCAAAAAGGGGAAACAACCTAAATGTTCATCAAC1520.1154532680110896No Hit
TATTTATGCAGTAGGTTTTAATATCGCCATAAGTGTCTTCATTACAAGCAA1490.11317458508981809No Hit
GGAATGGAATGGAATGGAATAGAATGGAATGGAATGGAATCGAATGGAATG1480.11241502411606093No Hit
GTATGTACCTATGGGACAGATTAAGATGACTAACAAGTCCAACAAAGCAGA1480.11241502411606093No Hit
GGTAGGAAATGAACATATGTTTTGAAGCTTTTGTAACTGATCTTCTACTCA1470.11165546314230373No Hit
AATAGGTGTCTATTTACAAACTCTCTTGTGTCTCTCAGAGTAGTTATTTTC1460.11089590216854658No Hit
TTTTTGTTTTTGGTTTATTTTTGTTGTTGTTGTTGTTGCATGAAATCTTAA1430.10861721924727506No Hit
CTTGATTACAATTTGGGGGTCTTTGAATCTTGATTACTTAGCTATTTTTCC1430.10861721924727506No Hit
CATTAATCATTCCCAAACTCCTGTCACTCTTCCAAAACATAGAAGAAGAGA1420.1078576582735179No Hit
ATTGGTAGGAGATTTCATTGTCCTTCTTATTTTTCCTTCAAGGTTATAAAG1390.10557897535224639No Hit
AATGAATGTTGTGTTTGGTAATTGTAATCACACCATAAAGTACCTGACATA1360.10330029243097491No Hit
GATAAAAAGGGATACCCATGTCTCTATTAACAGCTTTATCTCTTTCTACAG1360.10330029243097491No Hit
TTGTAAATATTGAGCAAATTGCTCAATTTTTTTATCGATGATTTTCACTTG1350.10254073145721772No Hit
TCATAAAACACACATATTTTGTAGAGTGAGAAGCAAGAAAGATTTGATTCA1350.10254073145721772No Hit
GAAAAAAACTGGAGTTACAGATTTGAGATATTCAAGGAAAGAAAATGTGAG1350.10254073145721772No Hit
AAGCAAGCTAAAGAGAATATGACCATTTTATGACATTTCAAACATTCTCAA1340.10178117048346055No Hit
CAAATAGAGACACCAATTGTAAAACTTAAGGAATCCTGGTCCCCAAAATAC1340.10178117048346055No Hit
ACTATAAGAACTGCACTTTTGTCCTTATTTTTTTCCTCCATTTAAGCCTCA1330.1010216095097034No Hit
CTTTTTGAAAGACAAAAAGAAAATGATCTTTGAGAAAGAAATACCAGCTTT1320.10026204853594624No Hit
ATTGATGATGGTTATTTTTGATTCCATTTGATGATGATTACATTCGATTCC1320.10026204853594624No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGATG253.8646147E-545.014443
ACGCCTA207.012564E-444.9973442
GTCTGGC207.012564E-444.9973442
TTTACGG207.012564E-444.9973423
TCCGAAC207.012564E-444.9973442
CCAGCTA207.012564E-444.9973412
CGTCTTA207.012564E-444.9973444
CTAAGGC207.012564E-444.9973440
TACGGAC207.012564E-444.9973425
CGCCTAT207.012564E-444.9973443
GACCAGA207.012564E-444.9973445
TCCAGGG207.012564E-444.9973440
GCTTAAA207.012564E-444.9973413
CGGGCCA207.012564E-444.9973442
TTGCGAT207.012564E-444.9973427
CGAATGT302.151708E-644.9973421
GGTGCTT207.012564E-444.9973419
GCCTATT207.012564E-444.9973444
GCAGGAT207.012564E-444.9973414
TTACGGA207.012564E-444.9973424
Produced by FastQC (version 0.11.2)