FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0120_pd_72.3510000004643b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0120_pd_72.3510000004643b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences156835
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC77904.967003538750917No Hit
TTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTCT11380.7256033410909555Illumina PCR Primer Index 11 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGT7150.45589313609844745No Hit
ATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTCTTC3380.21551311888290242Illumina PCR Primer Index 11 (95% over 23bp)
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACCTGTCTCT2700.1721554499952179No Hit
CATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAAT2160.13772435999617433No Hit
GGGCTAAACCATGCACCGAAGCTGCGGCAGCGACGCTTATGCGTTGTTGGG1940.12369687888545286No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTT1850.11795836388561226No Hit
TATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTCTT1850.11795836388561226Illumina PCR Primer Index 11 (95% over 22bp)
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTC1780.11349507444129181No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTTT1770.11285746166353172No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGCTGTCTCTTATACACATCT1630.1039308827748908No Hit
CACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCT1620.10329326999713073No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGA207.0156215E-444.99872621
TGCCGTC11500.036.78156745
GCCGTCT2000.035.99897845
ATGCCGT12050.035.10273744
CGAGACG12250.035.08063523
CGTATGC12300.034.93803441
CACGAGA12300.034.93803421
CGCTATA12200.034.8555731
GTATGCC12150.034.81382842
TATCTCG12350.034.79658536
CCACGAG12350.034.79658520
ACGACGC12350.034.79658527
TCGTATG12300.034.7551140
TATGCCG12250.034.713343
ATCTCGT12400.034.65627737
AGACGAC12400.034.65627725
CTCGTAT12350.034.61440739
GCTATAT12300.034.5721932
CCCACGA12450.034.51709419
ATATCTC12450.034.51709435
Produced by FastQC (version 0.11.2)