FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0120_pd_71.35100000046411.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0120_pd_71.35100000046411.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences529523
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC56251.0622768038404375No Hit
TCTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGT6900.130305954604427No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTT6830.12898401013742558No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTATA6800.037.71861331
TATGCCG6700.037.27416243
TGCCGTC6750.036.9980645
ATCTCGT6950.036.90453737
ACGCTAT6950.036.90453730
TCGTATG6900.036.84589440
ACGACGC6900.036.84589427
TATCTCG6900.036.51982536
GACGCTA7100.036.44175329
CGAGACG6950.036.26051323
CGTATGC7050.036.0619441
CTCGTAT7050.036.0619439
ACGAGAC7050.035.74618522
AGACGAC7200.035.31064625
ATGCCGT7200.034.6856844
TCTCGTA7350.034.59002338
CGACGCT7400.034.35630428
GAGACGA7350.034.28715524
CACGAGA7450.034.1289521
GTATGCC7600.033.15615542
Produced by FastQC (version 0.11.2)