H23LNBCXX_l02n01_bir_0120_pd_70.351000000463f6.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H23LNBCXX_l02n01_bir_0120_pd_70.351000000463f6.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	155306
Sequences flagged as poor quality	0
Sequence length	51
%GC	43

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC	12502	8.049914362613164	No Hit
TCTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGT	5594	3.6019213681377407	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACA	1943	1.2510785159620361	No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTT	1139	0.7333908541846419	No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC	494	0.31808172253486666	No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAA	480	0.3090672607626235	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCG	469	0.30198446937014667	No Hit
TTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTCT	408	0.26270717164823	Illumina PCR Primer Index 11 (95% over 21bp)
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACCTGTCTCT	391	0.25176103949622036	No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTTT	342	0.2202104232933692	No Hit
GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA	336	0.21634708253383644	No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA	317	0.2041131701286492	No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC	305	0.19638648860958366	No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACCTGTCTCTTATACAC	296	0.19059147747028446	No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC	257	0.1654797625333213	No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGA	254	0.16354809215355492	No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTC	208	0.13392914633047018	No Hit
CATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATC	200	0.12877802531775978	No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACCTGTCTCTTATAC	198	0.12749024506458217	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCC	192	0.12362690430504938	No Hit
AAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCACGA	183	0.1178318931657502	No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACCTGTCTCTT	179	0.11525633265939499	No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA	159	0.10237853012761901	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCCGTC	1525	0.0	42.490284	45
GCCGTCT	80	0.0	36.561195	45
GTTAGTT	25	0.0021018824	35.998714	19
ATGCCGT	2160	0.0	29.998926	44
CGTATGC	2265	0.0	28.90625	41
TATGCCG	2250	0.0	28.898966	43
CGCTATA	2250	0.0	28.898966	31
TCGTATG	2250	0.0	28.898966	40
ATCTCGT	2230	0.0	28.85547	37
TATCTCG	2230	0.0	28.85547	36
CGAGACG	2270	0.0	28.84258	23
GCTATAT	2255	0.0	28.834888	32
CTATATC	2240	0.0	28.827093	33
ACGCTAT	2250	0.0	28.79897	30
CTCGTAT	2235	0.0	28.790918	39
CACGAGA	2275	0.0	28.77919	21
GACGACG	2260	0.0	28.771095	26
ACGACGC	2260	0.0	28.771095	27
TATATCT	2240	0.0	28.72665	34
ACGAGAC	2280	0.0	28.716076	22

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content