FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0120_pd_65.35100000046356.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0120_pd_65.35100000046356.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences237168
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC149776.314932874586791No Hit
TCTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGT33421.4091277069419146No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCG5460.23021655535316737No Hit
TTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTCT4580.19311205558928693Illumina PCR Primer Index 11 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTGCA207.0245477E-444.9966288
GCCGTCT502.1827873E-1144.99662845
TGCCGTC15350.043.53093745
GTGGTGT250.00210234236.004896
CGAGTGG250.002104519435.997325
TTGCGTC250.002104519435.997328
CCTTGAC250.002104519435.997330
ATCTGGG250.002104519435.997311
ATGCCGT18700.035.73261644
TATGCCG19450.034.35474843
CTCGTAT19650.034.23407739
TATCTCG19750.034.17465236
CGTATGC19700.034.1471941
CGACGCT19700.034.1471928
GACGCTA19700.034.1471929
CGCTATA19750.034.06073831
ATCTCGT19850.034.00248737
ACGCTAT19800.033.97472430
CGAGACG19800.033.97472423
GTATGCC19700.033.9187842
Produced by FastQC (version 0.11.2)