FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0120_pd_62.35100000046305.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0120_pd_62.35100000046305.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences329230
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTC90522.7494456762749446No Hit
TCTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGT17210.5227348662029585No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACCTGTCTCT15690.476566534033958No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTT9130.27731373204143No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACA7890.23965009264040335No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGCTGTCTCTTATACACATCT6500.19743036782796222No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACCTGTCT6110.18558454575828448No Hit
TCATTAACCTATGGATTCAGTTAATGATAGCTGTCTCTTATACACATCTCC5880.17859854812744888No Hit
CACTATCATTAACTGAATCCATAGGTTAATGACTGTCTCTTATACACATCT5800.17616863590802784No Hit
CTATCATTAACTGAATCCATAGGTTAATGACTGTCTCTTATACACATCTCC5600.17009385535947513No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA4790.14549099413783678No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC4730.14366855997327097No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACCTGTCTCTT4680.1421498648361328No Hit
AACCCAGTGTGTTTCGACACACTATCATTAACTGAATCCATAGGTTAATGA4580.13911247456185646No Hit
GTGTTTCGACACACTATCATTAACTGAATCCATAGGTTAATGACTGTCTCT4510.136986301369863No Hit
TCGACACACTATCATTAACTGAATCCATAGGTTAATGACTGTCTCTTATAC3920.11906569875163259No Hit
ACCCAGTGTGTTTCGACACACTATCATTAACTGAATCCATAGGTTAATGAC3830.1163320475047839No Hit
CATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAAT3820.11602830847735625No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA3800.11542083042250098No Hit
GTACGGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGA3760.11420587431279046No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACCTGTCTCTTA3730.11329465723050754No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCC3730.11329465723050754No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACA3690.11207970112079702No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACTGTCTCTTAT3670.11147222306594175No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTTT3560.10813109376423777No Hit
CACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCT3540.1075236157093825No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA3410.10357500835282325No Hit
GTTTCGACACACTATCATTAACTGAATCCATAGGTTAATGACTGTCTCTTA3370.10236005224311272No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAGT3350.10175257418825745No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGACTA207.014032E-445.01556
TGCCGTC9700.042.6756645
CGACTGT552.739398E-936.81408336
ATGCCGT11500.035.9959944
TATGCCG11850.034.93281643
GGCGAAT11900.034.7860432
ATCTCGT12000.034.68363637
CGTATGC12000.034.68363641
CTCGTAT12150.034.25544439
AATCTCG12100.034.21106336
TCGTATG12200.034.11505540
GCGAATC12150.034.0702833
CACGAGA12250.033.97580721
CGAGACC12200.033.9306523
CGGGGAC401.5503003E-533.7718854
CCACGAG12350.033.700720
CGAATCT12300.033.6547934
GTATGCC12350.033.5185342
AAGGCGA12350.033.33636530
TCTCGTA12550.033.16363538
Produced by FastQC (version 0.11.2)