FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0120_pd_61.351000000462ea.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0120_pd_61.351000000462ea.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69783
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTC27343.9178596506312426No Hit
TCTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGT4830.6921456515197111No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC2250.3224280985340269No Hit
TTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTCT1730.24791138242838515No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACCTGTCTCT1300.18629179026410445No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTT1290.1848587764928421No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACA1250.17912672140779273No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGC950.13613630826992248No Hit
CATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAAT840.12037315678603672No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA840.12037315678603672No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCCAA206.9905E-444.99641411
TGCCGTC4000.037.12204445
GAACACT250.002094415735.997136
TGGCTTT250.002094415735.9971319
ATCGCCG250.002094415735.9971319
GCCGTCT653.5470293E-1034.61262545
CGAGACC4700.032.550623
ATGCCGT4600.032.28003744
CACGAGA4750.032.20796221
GGCGAAT4750.032.20796232
ACGAGAC4750.032.20796222
ATCTCGT4700.032.07191537
GCGAATC4700.032.07191533
CCACGAG4850.032.0077620
TCTCGTA4750.031.73431438
CGAATCT4750.031.73431434
ACCAAGG4700.031.59322727
CGTATGC4850.031.54387741
GTATGCC4850.031.54387742
CTCGTAT4800.031.4037539
Produced by FastQC (version 0.11.2)