FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0120_pd_58.3510000004628d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0120_pd_58.3510000004628d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences344059
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTC158654.611127742625538No Hit
TCTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGT28450.826893061945771No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC14020.4074882505616769No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACC7450.21653262957806654No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCA7160.20810384265489348No Hit
CTATCATTAACTGAATCCATAGGTTAATGACTGTCTCTTATACACATCTCC6400.18601460795968133No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACCTGTCTCT6290.1828174818853743No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCAC6190.1799110036360043No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGT5600.16276278196472116No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGC5380.1563685298161071No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCG5220.1517181646171151No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAG4190.12178143864860387No Hit
TCATTAACCTATGGATTCAGTTAATGATAGCTGTCTCTTATACACATCTCC4060.11800301692442286No Hit
GCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATA3810.1107368213009978No Hit
CACTATCATTAACTGAATCCATAGGTTAATGACTGTCTCTTATACACATCT3710.10783034305162777No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGCTGTCTCTTATACACATCT3530.10259868220276175No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGCA302.162109E-644.99680326
TGCCGTC16500.042.5424345
GCCGTCT700.041.78274545
ATTGACG403.450732E-739.37220417
TACCCGC356.238577E-638.5686925
CCGGCTA301.1387362E-437.49733742
CGGCTAA301.1387362E-437.49733743
CGATGTG301.1387362E-437.49733742
TATCTCG250.002099793436.018384
GGCGAGA250.00210279336.0079086
TACGAGG250.002105795835.997448
ATGCCGT19900.035.27387644
TATGCCG20500.034.2414743
CGAGACC20600.034.18446423
GGCGAAT20350.034.05163632
CGTATGC20500.034.02197341
GACCAAG20700.034.0193226
GCGAATC20400.033.96817433
CGAATCT20500.033.91222434
GAGACCA20850.033.88248424