FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0120_pd_57.35100000046263.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0120_pd_57.35100000046263.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22577
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTC263311.662311201665412No Hit
TCTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGT2721.2047659122115428No Hit
TTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTCT2341.036453027417283No Hit
ATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTCTTC1310.5802365238960003RNA PCR Primer, Index 46 (95% over 22bp)
TATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTCTT660.29233290516897725RNA PCR Primer, Index 46 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCG650.2879036187270231No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC3150.037.85714345
CCGAGCC3500.036.014
TCCGAGC3500.036.013
CTCCGAG3500.036.012
GAGCCCA3450.035.86956416
CGAGCCC3450.035.86956415
TCTCCGA3600.035.62511
ATCTCCG3650.035.13698610
GCCCACG3550.034.85915418
AGCCCAC3600.034.37517
CCACGAG3550.033.5915520
CACGAGA3550.033.5915521
CCCACGA3550.033.5915519
ACGAGAC3550.033.5915522
ATGCCGT3550.033.5915544
CGAGACC3550.033.5915523
AGACCAA3550.033.5915525
GAGACCA3550.033.5915524
GTATGCC3600.033.12542
ATCTCGT3600.033.12537
Produced by FastQC (version 0.11.2)