FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0120_pd_56.3510000004624a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0120_pd_56.3510000004624a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences253870
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC181067.131996691219916No Hit
TCTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGT35161.3849608067120969No Hit
TTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTCT14000.5514633473825187No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACCTGTCTCT10500.413597510536889No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTT7010.2761255760822468No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC4620.18198290463623112No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC4540.17883168550833103No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCG4480.17646827116240596No Hit
TCATTAACCTATGGATTCAGTTAATGATAGCTGTCTCTTATACACATCTCC4330.17055973529759327No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACA4250.16740851616969316No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACCTGTCT3830.1508646157482176No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACCTGTCTCTT3760.148107299011305No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGCTGTCTCTTATACACATCT3290.12959388663489188No Hit
CTATCATTAACTGAATCCATAGGTTAATGACTGTCTCTTATACACATCTCC3220.12683656989797928No Hit
CACTATCATTAACTGAATCCATAGGTTAATGACTGTCTCTTATACACATCT3150.12407925316106669No Hit
GTACGGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGA2870.11304998621341632No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTTT2820.11108047425847875No Hit
ATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTCTTC2810.11068657186749124Illumina PCR Primer Index 4 (95% over 22bp)
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCC2740.10792925513057863No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTC2690.10595974317564108No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC2610.10280852404774096No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGACTGTCTCTTATAC2550.10044510970181589No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGGTA207.022891E-445.00000422
ATAACGC207.022891E-445.0000043
TGCCGTC20050.040.28678545
CGGGATA356.229073E-638.571434
GGGATAC301.1374181E-437.4999965
TAAAGGT250.002104024236.04
TGCGGGT250.002104024236.021
TAACGCA250.002104024236.04
CCCATAC250.002104024236.03
GCCGTCT1900.034.34210645
ATGCCGT23600.034.13135544
TATGCCG24100.033.42323743
TATCTCG24100.033.23651536
ATCTCGT24250.033.0309337
CTATCTC24250.033.0309335
CGTATGC24450.032.94478641
ACCTATC24400.032.92008233
CCTATCT24350.032.89527534
CACGAGA24700.032.88461721
CCACGAG24650.032.8600420
Produced by FastQC (version 0.11.2)