FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0120_pd_51.351000000461a8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0120_pd_51.351000000461a8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences169710
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC6694239.44493547816864No Hit
TCTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGT121547.1616286606564135No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCG104136.13576100406576No Hit
TTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTCT3440.2026987213481822No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC72000.044.56220645
GCCGTCT650.041.5381945
ATGCCGT85300.037.6140644
TATGCCG96000.033.42165843
CGTATGC96600.033.3305341
TCGTATG96650.033.31328240
GTATGCC96200.033.30539742
CTCGTAT96500.033.271839
TCTCGTA96500.033.24848638
TATCTCG96350.033.23019436
ATCTCGT96600.033.2140737
CTATCTC96900.033.1112435
CCTATCT96950.033.07095334
CGAGCCC97900.033.02583315
CGAGACG97700.033.0243523
ACGAGAC97700.033.0243522
ACCTATC97100.033.01986733
GAGCCCA97850.033.0197116
AACCTAT97200.033.00904532
GCCCACG97950.033.00897218