FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0120_pd_50.3510000004618e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0120_pd_50.3510000004618e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences383343
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC348189.08272747904618No Hit
TCTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGT171624.476930581750547No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACA17930.46772733557153773No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCG16910.44111931090433365No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTT16340.4262501206491315No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACCTGTCTCT10290.2684280135544408No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC9800.2556457271947055No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC8030.20947297850749852No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC7750.20216881487336408No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAA5910.15417002527762344No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA5440.1419094648917549No Hit
GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA5140.13408357528375373No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTC4730.12338819281948542No Hit
TTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTCT4150.1082581395773498No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTTT4060.10591037269494943No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACCTGTCTCTTATACAC4060.10591037269494943No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC39800.043.92498445
GAACCGC250.002105570235.9992732
ATGCCGT57150.030.5899344
TTAGAGT300.005140258530.0033054
AACCGCT300.00514353929.99939233
GCGAATG300.00514353929.99939211
GTGGCAC300.00514353929.99939222
ACCTACT453.4853216E-529.9993927
TGCCGAG300.00514353929.99939240
TATGCCG58800.029.7315443
CGTATGC59250.029.61965241
ATCTCGT59300.029.51879337
TCGTATG59500.029.49520140
CTCGTAT59350.029.49392539
TCTCGTA59450.029.4821638
CGATAAC59300.029.4429128
ACCTATC59350.029.41810633
GTATGCC59500.029.38175842
GATAACC59450.029.36862229
TATCTCG59450.029.36862236
Produced by FastQC (version 0.11.2)