FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0120_pd_49.35100000046164.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0120_pd_49.35100000046164.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences207321
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC6466931.192691526666376No Hit
TCTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGT2846413.729434066013575No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCG38271.8459297418013614No Hit
TTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTCT12770.6159530390071435No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATAGT207.0200855E-444.9997564
TGCCGTC68750.043.49431245
GCCGTCT2300.037.1737145
GTATCTA250.002100701236.008491
CTGGTAG250.002103190935.9998059
TTTATAC754.7293724E-1132.9998253
ATGCCGT98700.030.2961944
GGCAATA300.005137780729.99983818
CGTCTTC300.005137780729.99983845
TGTATCA300.005137780729.9998382
TCAGGGA300.005137780729.99983832
GGTTCTG300.005137780729.99983814
AGGGACT300.005137780729.99983820
ATCTCGT102700.029.2476537
TCTCGTA102950.029.24219338
TATCTCG102450.029.23117636
CTCGTAT103000.029.20615439
CTATCTC102550.029.20267135
CCTATCT102550.029.20267134
CCCACGA103850.029.162119
Produced by FastQC (version 0.11.2)