H23LNBCXX_l02n01_bir_0120_pd_45.351000000460df.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H23LNBCXX_l02n01_bir_0120_pd_45.351000000460df.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	41014
Sequences flagged as poor quality	0
Sequence length	51
%GC	45

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTC	6287	15.32891207880236	RNA PCR Primer, Index 46 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGT	1648	4.018140147266787	No Hit
TTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTCT	352	0.8582435266006729	RNA PCR Primer, Index 18 (95% over 21bp)
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACA	303	0.7387721265909202	No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTT	188	0.45838006534354125	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCG	102	0.24869556736724047	No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC	81	0.19749353879163212	No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCG	79	0.19261715511776467	No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTTT	75	0.18286438777002975	No Hit
TATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTCTT	60	0.1462915102160238	RNA PCR Primer, Index 46 (95% over 23bp)
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA	56	0.13653874286828888	No Hit
ATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTCTTC	56	0.13653874286828888	RNA PCR Primer, Index 46 (95% over 24bp)
CATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATC	53	0.12922416735748768	No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC	51	0.12434778368362023	No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGC	45	0.10971863266201784	No Hit
GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA	44	0.10728044082508412	No Hit
GTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCC	43	0.1048422489881504	No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACTGTCTCTTA	42	0.10240405715121666	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
TGCCGTC	875	40.62857	45
GCCGTCT	85	37.058823	45
ACCATTC	1040	34.83173	27
GACCATT	1035	34.782608	26
CGAATCT	1040	34.615383	34
CCGAATC	1040	34.615383	33
GAGACCA	1010	34.5297	24
GAATCTC	1050	34.499996	35
CGAGACC	1025	34.463413	23
TCCGAAT	1045	34.44976	32
ACGAGAC	1020	34.411766	22
AGACCAT	1020	34.411766	25
ATCTCGT	1060	34.38679	37
ATGCCGT	1035	34.347828	44
AATCTCG	1055	34.33649	36
ATTCCGA	1050	34.285713	30
CACGAGA	1025	34.2439	21
CCCACGA	1025	34.2439	19
TTCCGAA	1045	34.23445	31
TATGCCG	1060	34.17453	43

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content