H23LNBCXX_l02n01_bir_0120_pd_44.351000000460c2.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H23LNBCXX_l02n01_bir_0120_pd_44.351000000460c2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	355459
Sequences flagged as poor quality	0
Sequence length	51
%GC	41

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTC	14486	4.075294197080394	RNA PCR Primer, Index 46 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGT	3002	0.8445418458950259	No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC	2031	0.5713739137284469	No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTT	1498	0.42142694375441336	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACA	1418	0.39892083193842326	No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACCTGTCTCT	1124	0.31621087101465994	No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACC	1107	0.31142832225376205	No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCA	1002	0.28188905049527513	No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGC	826	0.23237560450009706	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAGT	781	0.21971591660360268	No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCAC	777	0.21859061101280317	No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGT	726	0.20424296473010953	No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAG	708	0.19917908957151179	No Hit
GTGTTTCGACACACTATCATTAACTGAATCCATAGGTTAATGACTGTCTCT	594	0.16710788023372597	No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCA	568	0.1597933938935292	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCG	516	0.1451644212131357	No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAA	507	0.14263248363383682	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCC	499	0.14038187245223782	No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGC	483	0.13588065008903982	No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA	480	0.13503667089594018	No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAAC	452	0.12715953176034367	No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGCTGTCTCTTATACACATCT	444	0.12490892057874467	No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAA	431	0.12125167740864627	No Hit
CACTATCATTAACTGAATCCATAGGTTAATGACTGTCTCTTATACACATCT	421	0.11843841343164754	No Hit
AAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGT	404	0.11365586467074966	No Hit
GCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATA	401	0.11281188547765002	No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA	399	0.11224923268225027	No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC	397	0.11168657988685053	No Hit
TCATTAACCTATGGATTCAGTTAATGATAGCTGTCTCTTATACACATCTCC	388	0.10915464230755163	No Hit
ACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGG	382	0.10746668392135239	No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA	370	0.10409076714895389	No Hit
CTATCATTAACTGAATCCATAGGTTAATGACTGTCTCTTATACACATCTCC	369	0.103809440751254	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCCGTC	1395	0.0	43.707767	45
GACCATG	30	1.13862945E-4	37.49836	7
CGTCCCG	30	1.13862945E-4	37.49836	12
GCGTCCC	30	1.13862945E-4	37.49836	11
AACGTAT	25	0.002105608	35.998425	37
ATGCCGT	1695	0.0	35.839138	44
TATGCCG	1770	0.0	34.32053	43
CCGAATC	1770	0.0	34.193417	33
CGTATGC	1780	0.0	34.12772	41
CGAATCT	1785	0.0	34.032124	34
ATCTCGT	1790	0.0	33.93706	37
GAGACCA	1810	0.0	33.93498	24
CGAGACC	1810	0.0	33.93498	23
AATCTCG	1795	0.0	33.717186	36
GTATGCC	1805	0.0	33.655037	42
GACCATT	1820	0.0	33.6249	26
CACGAGA	1830	0.0	33.564106	21
CTCGTAT	1810	0.0	33.562065	39
GAATCTC	1810	0.0	33.562065	35
CATTCCG	1820	0.0	33.50128	29

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content