Basic Statistics
Measure | Value |
---|---|
Filename | H23LNBCXX_l02n01_bir_0120_pd_43.351000000460a9.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 129491 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 49 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTC | 60521 | 46.7376111081079 | RNA PCR Primer, Index 46 (95% over 21bp) |
TCTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGT | 10582 | 8.17199650940992 | No Hit |
CTCTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCG | 5747 | 4.438146280436478 | No Hit |
TTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTCT | 232 | 0.17916303063533373 | RNA PCR Primer, Index 18 (95% over 21bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TGCCGTC | 6450 | 0.0 | 44.403168 | 45 |
ATGCCGT | 7505 | 0.0 | 38.16128 | 44 |
GCCGTCT | 80 | 0.0 | 36.55936 | 45 |
TATGCCG | 8070 | 0.0 | 35.517395 | 43 |
CGTATGC | 8145 | 0.0 | 35.411324 | 41 |
TCGTATG | 8135 | 0.0 | 35.399544 | 40 |
ATCTCGT | 8135 | 0.0 | 35.371887 | 37 |
GTATGCC | 8120 | 0.0 | 35.354107 | 42 |
AATCTCG | 8110 | 0.0 | 35.34222 | 36 |
TCTCGTA | 8150 | 0.0 | 35.33439 | 38 |
CCCACGA | 8190 | 0.0 | 35.326637 | 19 |
GCCCACG | 8210 | 0.0 | 35.295387 | 18 |
CTCGTAT | 8140 | 0.0 | 35.29488 | 39 |
CGAATCT | 8090 | 0.0 | 35.290543 | 34 |
CCACGAG | 8200 | 0.0 | 35.283558 | 20 |
GAATCTC | 8105 | 0.0 | 35.280746 | 35 |
ACGAGAC | 8190 | 0.0 | 35.271698 | 22 |
CCGAATC | 8095 | 0.0 | 35.268745 | 33 |
CACGAGA | 8210 | 0.0 | 35.267986 | 21 |
TCCGAAT | 8115 | 0.0 | 35.264996 | 32 |