FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0120_pd_43.351000000460a9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0120_pd_43.351000000460a9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences129491
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTC6052146.7376111081079RNA PCR Primer, Index 46 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGT105828.17199650940992No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCG57474.438146280436478No Hit
TTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTCT2320.17916303063533373RNA PCR Primer, Index 18 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC64500.044.40316845
ATGCCGT75050.038.1612844
GCCGTCT800.036.5593645
TATGCCG80700.035.51739543
CGTATGC81450.035.41132441
TCGTATG81350.035.39954440
ATCTCGT81350.035.37188737
GTATGCC81200.035.35410742
AATCTCG81100.035.3422236
TCTCGTA81500.035.3343938
CCCACGA81900.035.32663719
GCCCACG82100.035.29538718
CTCGTAT81400.035.2948839
CGAATCT80900.035.29054334
CCACGAG82000.035.28355820
GAATCTC81050.035.28074635
ACGAGAC81900.035.27169822
CCGAATC80950.035.26874533
CACGAGA82100.035.26798621
TCCGAAT81150.035.26499632