FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0120_pd_42.3510000004608f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0120_pd_42.3510000004608f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117964
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTC2788723.64026313112475RNA PCR Primer, Index 46 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGT116429.86911261062697No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCG12131.0282798141805973No Hit
TTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTCT4920.4170763961886677RNA PCR Primer, Index 18 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCG1200.10172595028991895No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC31800.043.65360645
GCCGTCT950.035.52464345
ATGCCGT43850.031.65757444
TATGCCG45300.030.64425343
TCTCGTA45250.030.62839138
CCCACGA45550.030.6242419
CCACGAG45550.030.6242420
CGAATCT44900.030.61659834
ATCTCGT45200.030.61249737
CACGAGA45500.030.6084521
ACGAGAC45500.030.6084522
AATCTCG45000.030.59855836
CTCGTAT45300.030.59458539
CCGAATC44950.030.5825433
GTATGCC45400.030.57675442
GCCCACG45550.030.57484818
CATTCCG45200.030.56272129
TCGTATG45350.030.56085640
TCCGAAT45000.030.54856132
AGCCCAC45600.030.54132317
Produced by FastQC (version 0.11.2)