FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0120_pd_41.35100000046059.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0120_pd_41.35100000046059.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences144146
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTC3521624.430785453637284RNA PCR Primer, Index 46 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGT131209.10188281325878No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCG14801.0267367807639478No Hit
TTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTCT8650.6000860238924424RNA PCR Primer, Index 18 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTCTTC1790.12417965118699097RNA PCR Primer, Index 46 (95% over 24bp)
TCTTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCG1760.12209842798273972No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAT207.002617E-445.0131876
TGCCGTC38550.043.0132445
GCCGTCT1650.034.0890745
AAGCTGA401.5480377E-533.759896
ATGCCGT52400.031.64428544
CCGAATC53850.030.83399233
TCTCGTA54000.030.83166738
CGAATCT53800.030.82082634
CTCGTAT53950.030.8185439
GAATCTC53750.030.80763835
ATTCCGA54000.030.79000330
TCCGAAT53900.030.76364732
AATCTCG53900.030.76364736
CGTATGC54000.030.74833941
TCGTATG54000.030.74833940
TTCCGAA53950.030.73513431
TATGCCG54000.030.70667543
CGAGACC54450.030.70082323
ACGAGAC54500.030.67265722
GCCCACG54450.030.65950418
Produced by FastQC (version 0.11.2)