FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0120_pd_4.35100000045bcb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0120_pd_4.35100000045bcb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences304924
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC170785.60073985648883RNA PCR Primer, Index 27 (95% over 21bp)
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA20150.6608204011491388No Hit
TCTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGT18190.5965420891763193No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC17460.5726016974721571No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA13440.44076556781361914No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA13040.42764754496202334No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12460.40862641182720943No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11640.38173446498143804No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9270.30401017958573284No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA8830.28958035444897745No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA8370.27449462816964226No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCA8200.26891946845771403No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7240.23743621361388412No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7180.23546851018614473No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGC7010.22989335047421652No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6840.2243181907622883No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6700.21972688276422978No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCC6540.21447967362359147No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAG6040.19808214505909671No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACC5920.19414673820361794No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA5660.18562002335008068No Hit
GTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5630.184636171636211No Hit
GGTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA5380.17643740735396363No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA4670.15315291679238105No Hit
GTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4480.14692185593787305No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCAC4460.14626595479529325No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGT4420.14495415251013366No Hit
ATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA4340.1423305479398145No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTC4030.13216408022982776No Hit
GTACGGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGA3910.128228673374349No Hit
GTATCAACGCAGAGTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAA3890.12757277223176924No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCG3830.12560506880402986No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGC3720.12199761251984101No Hit
GCAAGATGGCTCGGGGATGGAGGTCCACGCTAATCAGTTTTCTCCTCTTGT3570.11707835395049258No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAA3510.11511065052275321No Hit
ATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTCTTC3490.11445474938017342RNA PCR Primer, Index 27 (95% over 24bp)
GAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA3430.11248704595243406No Hit
TATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAA3420.11215909538114414No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTTT3370.11051934252469468No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACA3310.10855163909695531No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAAC3300.10822368852566541No Hit
TTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTCT3080.10100877595728773RNA PCR Primer, Index 38 (95% over 21bp)
CATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAAT3050.10002492424341802No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTCCG207.0279E-444.99639543
CTAATCA502.1827873E-1144.9963930
TGCCGTC19050.042.16197645
GGTCCAC556.002665E-1140.9058122
GCTCGGG556.002665E-1140.905819
AGGTCCA556.002665E-1140.9058121
GCAAGAT601.5279511E-1037.5216061
ATCAGTT601.546141E-1037.49699433
CTCGTAT21650.037.2025639
CGTATGC21700.037.1168441
ATGCCGT21700.037.1168444
TATCTCG21650.037.09864436
TTGCTAT21600.037.0803632
ATCTCGT21750.037.03151337
CTATCTC21750.037.03151335
ATTGCTA21650.036.99472431
TCGTATG21800.036.9465840
GACGAAT21900.036.88060426
ACGAATT21900.036.88060427
TATGCCG21850.036.8620343
Produced by FastQC (version 0.11.2)