FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0120_pd_39.35100000046015.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0120_pd_39.35100000046015.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences450595
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC131932.927906434825065No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGT16940.3759473584926597No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCC12660.2809618393457539No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA10480.232581364640087No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACA10460.23213750707397995No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTC9180.20373062284312965No Hit
CACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCT9080.20151133501259444No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTT8730.1937438276057213No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTTT8600.1908587534260256No Hit
CATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAAT8550.18974910951075802No Hit
GTACGGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGA8270.18353510358525948No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA7370.16356151311044284No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACA7340.1628957267612823No Hit
CATATTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAAT7320.16245186919517526No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGTG6390.14181249237119808No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATG6020.13360112739821792No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACCTGTCTCT5830.12938448052020107No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATAA5770.12805290782187995No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA5700.12649940634050533No Hit
CAGTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC5680.1260555487743983No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCC5620.12472397607607719No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTC5470.12139504433027443No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAA5400.11984154284889979No Hit
CACAATATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCT5380.11939768528279274No Hit
GTACAATACAGTGGCATCCATGTGCAAGATTCTAAAAATCAAGCATATAAT5340.11850997015057868No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAGT5060.11229596422508017No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCAT4850.1076354597809563No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT4550.10097759628935074No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC14600.041.4536445
ATGCCGT16750.036.13272544
CGAACAA250.002106210735.998415
TATGCCG17000.035.6013643
CACGAGA17200.035.57981521
TATCTCG16950.035.57364336
TGCGACC16900.035.5457629
ATCTCGT17050.035.4969637
GACTGCG17000.035.46901326
CGACCTA16950.035.44090731
CGTATGC17100.035.39316641
CTGCGAC17050.035.2330428
TCGTATG17250.035.21582840
GCGACCT17150.035.1587930
GACCTAT17150.035.1587932
CTCGTAT17300.035.11404839
GTATGCC17350.034.8831842
CTATCTC17300.034.85394335
CCACGAG17600.034.77118320
ACCTATC17450.034.68327733
Produced by FastQC (version 0.11.2)