H23LNBCXX_l02n01_bir_0120_pd_38.35100000045ffe.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H23LNBCXX_l02n01_bir_0120_pd_38.35100000045ffe.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	284485
Sequences flagged as poor quality	0
Sequence length	51
%GC	39

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC	18514	6.507900240785982	No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGT	4169	1.465455120656625	No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACCTGTCTCT	1432	0.5033657310578765	No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA	1174	0.4126755364957731	No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA	968	0.34026398579889977	No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA	954	0.3353428124505686	No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTT	865	0.3040582104504631	No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC	846	0.29737947519201363	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACA	742	0.26082218746155333	No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC	728	0.25590101411322214	No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGCTGTCTCTTATACACATCT	635	0.22321036258502205	No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	633	0.22250733782097476	No Hit
CACTATCATTAACTGAATCCATAGGTTAATGACTGTCTCTTATACACATCT	600	0.21090742921419406	No Hit
TCATTAACCTATGGATTCAGTTAATGATAGCTGTCTCTTATACACATCTCC	575	0.20211961966360265	No Hit
AAAAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGA	573	0.20141659489955532	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCG	546	0.19192576058491662	No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACCTGTCT	541	0.1901681986747983	No Hit
CTATCATTAACTGAATCCATAGGTTAATGACTGTCTCTTATACACATCTCC	498	0.17505316624778108	No Hit
TTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTCT	438	0.15396242332636167	Illumina Paired End PCR Primer 2 (95% over 21bp)
GGTATCAACGCAGAGTACTTTTTTTTTTCTGTCTCTTATACACATCTCCGA	433	0.15220486141624337	No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA	426	0.1497442747420778	No Hit
GTGTTTCGACACACTATCATTAACTGAATCCATAGGTTAATGACTGTCTCT	417	0.14658066330386488	No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA	403	0.14165948995553368	No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACCTGTCTCTT	400	0.14060495280946272	No Hit
GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA	393	0.1381443661352971	No Hit
AAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGC	392	0.13779285375327346	No Hit
AACCCAGTGTGTTTCGACACACTATCATTAACTGAATCCATAGGTTAATGA	362	0.12724748229256375	No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA	335	0.11775664797792502	No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA	328	0.11529606130375941	No Hit
GGTATCAACGCAGAGTACTTTTTTTTCTGTCTCTTATACACATCTCCGAGC	326	0.1145930365397121	No Hit
GGTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA	325	0.11424152415768846	No Hit
TCGACACACTATCATTAACTGAATCCATAGGTTAATGACTGTCTCTTATAC	325	0.11424152415768846	No Hit
AAAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAG	323	0.11353849939364113	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTCTGTCTCTTATACACATCTCCGAG	303	0.106508251753168	No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACCTGTCTCTTA	302	0.10615673937114435	No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGACTGTCTCTTATAC	296	0.10404766507900241	No Hit
GTGTTTCGACACACTATCATTAACTGAATCCATAGGTTAATGACTGTCCCC	289	0.10158707840483683	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGATA	30	2.155195E-6	45.013718	4
GTTACGG	20	7.0259476E-4	44.997894	6
CGCACCA	20	7.0259476E-4	44.997894	30
TGCCGTC	1910	0.0	43.230953	45
CACCAAG	80	0.0	36.56079	7
AACACCA	85	0.0	34.422253	5
ATGCCGT	2480	0.0	33.29481	44
CTCGTAT	2520	0.0	32.944885	39
CGACCTA	2530	0.0	32.903595	31
TATCTCG	2510	0.0	32.896862	36
ATCTCGT	2520	0.0	32.855602	37
CTGCGAC	2520	0.0	32.855602	28
TGCGACC	2520	0.0	32.855602	29
GACTGCG	2520	0.0	32.855602	26
CGTATGC	2535	0.0	32.8387	41
CGAGACT	2545	0.0	32.709667	23
TATGCCG	2525	0.0	32.701435	43
GCGACCT	2540	0.0	32.685474	30
ACCTATC	2540	0.0	32.685474	33
TCGTATG	2540	0.0	32.685474	40

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content