FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0120_pd_37.35100000045fe1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0120_pd_37.35100000045fe1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73937
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC33854.5782219998106495No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGT9451.2781151520889407No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACA3180.43009589244897684No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTT2560.3462407184494908No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC2300.31107564548196437No Hit
TTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTCT2000.2705005612886647Illumina Paired End PCR Primer 2 (95% over 21bp)
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACCTGTCTCT1170.15824282835386883No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGC1100.14877530870876557No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCA1070.1447178002894356No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA900.1217252525798991No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA860.11631524135412581No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCG800.10820022451546586No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA770.1041427160961359No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC750.10143771048324926No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC3250.044.2850445
CTACTGG250.002071886836.079241
TACTGGA250.002085739736.0303542
TAGTACT250.002085739736.0303544
GATAAAG250.002092693536.00595528
TTATCAG250.002092693536.00595518
ATCTCGT4700.030.6433737
TATCTCG4700.030.6433736
ATGCCGT4700.030.62263744
GACCTAT4650.030.48891632
GCGACCT4750.030.32080830
CGACCTA4750.030.32080831
CTGCGAC4750.030.32080828
TATGCCG4750.030.30029543
CGTATGC4850.030.13922341
GAAGAGC300.00509542830.0252936
CTATCTC4800.030.00496535
TGCGACC4800.030.00496529
AAGGCGA300.005112352330.00496530
TGAGCGT300.005112352330.00496527
Produced by FastQC (version 0.11.2)