FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0120_pd_35.35100000045fae.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0120_pd_35.35100000045fae.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences137149
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC7157252.18557918759888No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGT1668312.164142647777235No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCG70005.103938052774719No Hit
TTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTCT2810.20488665611852805Illumina Paired End PCR Primer 2 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCAAAT207.0132234E-444.99781429
TGCCGTC72900.044.34969345
GTATGCT301.13501046E-437.4981842
CCTTATA250.002097396636.0113831
ATGCCGT90550.035.7050644
GCCGTCT950.033.1562845
GCGACCT98400.032.9938230
ACCTATC98400.032.9938233
GACCTAT98400.032.9938232
GCCCACG98950.032.99233218
AGCCCAC99100.032.98779717
CGAGCCC99250.032.98328415
CGACCTA98450.032.97706631
CCGAGCC99350.032.9727314
TCCGAGC99400.032.95614613
TGCGACC98450.032.95421229
CCCACGA99000.032.95294619
ACGAGAC98950.032.94685722
TATCTCG98550.032.94360436
CTATCTC98550.032.94360435
Produced by FastQC (version 0.11.2)