FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0120_pd_34.35100000045f77.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0120_pd_34.35100000045f77.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences141138
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC2213915.68606611968428No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGT45973.257095891963893No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCG7140.505887854440335No Hit
TTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTCT3610.25577803284728423Illumina Paired End PCR Primer 2 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG207.001633E-445.0138246
AAACGAT207.001633E-445.0138246
ATCTAGC207.013877E-444.997878
TGCCGTC23750.043.5768945
ATGCCGT28500.036.31407544
TGCCTTG250.002101350335.998334
CCTGATT250.002101350335.99838
TATGCCG29200.035.4435343
GCCCACG29250.035.38294218
GTATGCC29250.035.38294242
GAGCCCA29250.035.38294216
ACCTATC29200.035.3664833
GCCGTCT702.0008883E-1135.35547345
CGTATGC29300.035.32256341
CCCACGA29300.035.32256319
GCGACCT29250.035.30602330
CTCGTAT29250.035.30602339
TCTCGTA29250.035.30602338
CGAGCCC29450.035.2954515
AGCCCAC29350.035.26238617
Produced by FastQC (version 0.11.2)