FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0120_pd_33.35100000045f5e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0120_pd_33.35100000045f5e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences181604
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC115766.374308935926522No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGT24601.3545957137507985No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC4160.2290698442765578No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCG3960.21805687099403095No Hit
TCATTAACCTATGGATTCAGTTAATGATAGCTGTCTCTTATACACATCTCC3110.17125173454329198No Hit
CACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCT2260.12444659809255301No Hit
CTATCATTAACTGAATCCATAGGTTAATGACTGTCTCTTATACACATCTCC2220.12224400343604767No Hit
CATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAAT2100.11563621946653158No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCA2090.11508557080240522No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTTT2020.11123103015352086No Hit
TTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTCT1840.1013193541992467Illumina Paired End PCR Primer 2 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACCGA207.019069E-444.9980747
CTCTAGG207.019069E-444.99807412
TGCCGTC11650.042.4874545
TCTCTAG301.1364274E-437.49839411
TAGCACA301.1364274E-437.4983944
GAGTTGT250.0021000536.00837335
CTACGAA250.002102891435.9984611
GGAACCG250.002102891435.998466
CTAGGCC250.002102891435.9984614
TGAGAGC250.002102891435.998466
ATAGCAC401.553825E-533.7485583
ATGCCGT14900.033.2200544
CACGAGA15250.032.9002321
CCCACGA15300.032.79271319
CCACGAG15300.032.79271320
TATCTCG15150.032.67186736
CGACCTA15300.032.6546531
GCGACCT15400.032.58874530
TGCGACC15400.032.58874529
CTGCGAC15400.032.57977328
Produced by FastQC (version 0.11.2)