FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0120_pd_28.35100000045ec8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0120_pd_28.35100000045ec8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences296998
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTC4922016.572502171731795No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGT89092.9996834995521855No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCG13400.45118148943763936No Hit
TTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTCT9960.33535579364170803RNA PCR Primer, Index 40 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTCTTC2980.10033737600926605RNA PCR Primer, Index 40 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGGTAC207.014859E-445.012974
GCAACGG207.0265116E-444.997819
TGCCGTC52950.043.21319645
ATGCCGT63500.036.0336844
CGTAAAT250.002099885436.016442
TACAGCG250.002105100835.998257
ACGAGAC65100.035.1480622
CACGAGA65250.035.13622321
CGAGACC65000.035.13290423
GCCCACG65550.035.07838418
AGCCCAC65500.035.07081217
GAGACCT65000.035.0636824
TATGCCG65300.035.0404143
CCCACGA65700.035.0325419
ATCTCGT65150.035.01748337
CCGAGCC65600.035.01735314
AATCTCG65100.035.0098236
CCACGAG65550.035.00973520
CGTATGC65500.035.00211341
TCTCGTA65400.034.9868338
Produced by FastQC (version 0.11.2)