FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0120_pd_27.35100000045eaf.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0120_pd_27.35100000045eaf.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences294196
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTC118314.02146868074345No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGT42011.4279595915648071No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC9310.31645569620253167No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACA8980.30523868441447194No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTT6370.21652231845436376No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC5340.18151164529769268No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCG5290.1798120980570776No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCA4770.16213680675468056No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC4740.1611170784103115No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACCTGTCTCT3940.1339243225604699No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGC3890.1322247753198548No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACC3790.12882568083862458No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA3710.12610640525364045No Hit
GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA3610.12270731077241023No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCAC3130.10639165726250527No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAG3050.1036723816775211No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAA3050.1036723816775211No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC12600.043.38990445
GCCGTCT403.4483674E-739.37232245
ACTGGGC352.8164344E-432.1406744
ATGCCGT17350.031.51082444
TATGCCG18200.030.03916743
ACTGCGA300.00514281529.99796126
CGTATGC18300.029.99796141
TCGTATG18400.029.8349340
GTATGCC18400.029.71265642
AATCTCG18450.029.63213236
ATCTCGT18600.029.51412437
GCAGAAT18300.029.50619132
CGAGACC18500.029.43043123
GACCTTG18400.029.34583326
CCTTGCA18400.029.34583328
CACGAGA18750.029.27800821
CTTGCAG18450.029.26630229
TGCAGAA18600.029.15124531
CTCGTAT18850.029.12268839
CCACGAG18850.029.12268820
Produced by FastQC (version 0.11.2)