FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0120_pd_26.35100000045e85.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0120_pd_26.35100000045e85.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences144548
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTC117758.146082962061046No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGT29222.0214738356808812No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCG4510.3120070841519772No Hit
TTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTCT1810.1252179206907048RNA PCR Primer, Index 40 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTCCG207.007489E-445.006925
TGCCGTC12750.044.10916545
TTGTCGT250.002099442336.0055422
CTACACC250.002099442336.005544
TACTACA250.002099442336.005542
GAGTACG458.643128E-734.99327511
AGTACGG458.643128E-734.99327512
ATGCCGT16300.034.50256744
GTCATCA401.5532232E-533.743519
GAGACCT16950.033.58923724
AATCTCG16650.033.5186736
CGTATGC16850.033.50987641
GCCCACG17000.033.49044418
CACGAGA17000.033.49044421
CGAGACC17000.033.49044423
GACCTTG17000.033.49044426
ATCTCGT16800.033.48729337
TATGCCG16800.033.47570843
CCTTGCA16950.033.45647428
AGCCCAC16950.033.44489717
Produced by FastQC (version 0.11.2)