FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0120_pd_25.35100000045e6b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0120_pd_25.35100000045e6b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences143357
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTC3360823.443570945262525No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGT1630111.37091317480137No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCG22991.6036886932622754No Hit
TTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTCT5600.39063317452234636RNA PCR Primer, Index 40 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCAG207.01262E-445.043
TGCCGTC37250.043.4295345
GCCGTCT1250.037.845
TAAGTGA250.002100975336.04
ATGCCGT53450.030.22450844
GTATGCA300.005132416330.00000242
GTCAAAC300.005132416330.00000215
TTTATAC300.005132416330.0000023
GTAGTTC300.005132416330.00000237
ATCTCGT57000.028.65789437
TCTCGTA56950.028.64354938
CGTATGC56950.028.6040441
CTCGTAT57050.028.59333839
AATCTCG56900.028.5896336
CCACGAG57100.028.56830220
TATGCCG56600.028.54240243
GTATGCC56600.028.54240242
GCCCACG57000.028.53947318
CCCACGA57100.028.52889819
GAGCCCA57050.028.5144616
Produced by FastQC (version 0.11.2)